The Pharmacological Study On Exopolysaccharide Of Aphanothece Halophytical

Multiplicative biological function, especially its remarkable immunity, makes polysaccharide a hotspot of immunity pharmacology of Chinese traditional medicine. In order to put forward some further views and suggestions on the research of the new marine drugs, investigations have been made into the effects of EPAH on the immunity regulation, anti-tumour, anti-aging and anti-virus. For the sake of studying the effect of the immunity regulation of EPAH, observations were performed depending on the following experiments: the delayed type hypersensitivity(DTH), changes of total T-cell count of spleen in vitro, capability of spleen's T-cell secreting interleukin-II in vitro, activity of peritoneal macrophage producing interleukin-I in vivo, the production of NO secreted by cultured peritoneal macrophages, EPAH influencing the viability of peritoneal macrophages, the macrophages of the abdominal cavity of mice phagocytosing the erythrocytes of chickens , mononuclear phagocytes swallowing Congo Red,NK cell killing the target cells(Hela), and the serum producing the hemolysin made with sheep red blood cell (SRBC). S₁₈₀-bearing mice were used as animal model,and S180 tumour was inoculated in oxter of forelimb or abdominal cavity of mice separately. The inhibitory effects of EPAH on the transplanted tumour cells of S180 in mice were investigated by means of tumour weight,immune ability,the number of WBC, living time and the rate of the Erythrocytes to adhere tumour cells. Observation was also conducted on the anti-tumour action to SMMC7721 cells in vitro with MTT method. The anti-aging activity was evaluated by the survival time in the extreme environment, the swimming time and the weight of immune organs as well as the contents of malondialdehyde(MDA)in plasma, lipofuscin(Lf)in livers and brains, the activity of catalase(CAT), glutathione(GSH), and superoxide diamutase(SOD) in serum. Mouse pneumonia model induced by FM1 strain of influenza virus was used to observe the effects of EPAH on the levels of serous cytokine in serin, the lung index, the death rate and the virus titer in mouse lung. Ultrathin sections of mice lung tissues were also observed by indirect immunofluorescence assay and microscope. Results have been achieved as follows: 1. EPAH significantly enhanced the DTH in immuno-suppressed mice, decreased the DTH in immuno-increased mice, increased the secretion of IL-1 by macrophages and the secretion of IL-2 by spleen lymphocytes,and facilitated the lymphocyte proliferation in mice. 2. EPAH can enhance viability and NO production of peritoneal macrophages, and meanwhile decrease the level of nitric oxide in macrophages previously stimulated with LPS. 3. EPAH can improve the swallow function of the macrophage, promote the clearance rate of cogon red by mononuclear phagocytes, improve non-singular immunity of the body, increase NK cell activities of mice and maintain it in higher level, and promote the production of serum hemolysin. 4. EPAH can obviously inhibit the proliferation of the Sarcomal 180 (S180) cells and SMMC7721 cells in vivo or vitro,and enhance the rate of the Erythrocytes to adhere tumour cells. Furthermore, it can enhance the immunological function and recover the deduced number of WBC, and thus prolong the living time. 5. EPAH evidently enhanced the ability of anti-fatigue and the hypoxia tolerance ability, prolonged survival time in the extreme environment and enlarged the thymus and spleen of test mouse. EPAH also indicated significant activity in reducing the formation of MDA in serum, the Lf in brain and liver, and the increasing activity of SOD, CAT and GSH in serum. 6. EPAH can reduce immune pathological damages by controlling the excessive produce of TNF-a and raising the produce of IFN-γ, It can also decrease the mortality of mice and prolong the survive time. More importantly, the pathologic changes of the murine lung were significantly improved. Moreover, it can obviously decrease the heamoglutin titre of the virus, with comparison to the control murine group fed with ribavirin...