| Objective To study the cerebroprotection value of midazolam or ketamine alone and their combination on cerebral ischemia and reperfusion injury in rats.Methods 60 Wistar rats were randomly divided into 4 groups, which were group M, K, M-K and I-R. After intraperitoneally injected (i.p.) with hydrochloroaldehyde 350mg/kg for anesthesia, all animals' bilateral common carotid arteries were blocked for 30 minutes to establish a transient forebrain cerebral ischemia model. 5 minutes before reperfusion, animals were injected with midazolam 2mg/kg(M), ketamine 10mg/kg(K), midazolam 2mg/kg+ketamine 10mg/kg(M-K) or 0.9% normal saline 2ml(I-R) i.p. individually. 90 minutes after reperfusion, animals' brain tissue were took out under deep anesthesia, then the activity of lactic dehydrogenase(LDH) and superoxidized dismutase(SOD), the content of malondialdehyde(MDA) and the water rate of brain tissue were examined.Results There were no differences in the water rate of cerebral tissue in all therats. Compared to group I-R, the activity of LDH and the content of MDA were obviously decreased in every experimental group, while the activity of SOD were markedly increased in group M and M-K. In comparison to group K, the activity of LDH and the content of MDA were markedly decreased in group M-K, but the activity of SOD in M-K and M group was increased obviously. The variation ofConclusion In this study, we found that both midazolam and ketamine had acertain cerebroprotection value, and their combination group was superior to either of them alone. |
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