The Electrochemical Behavior Of Some Anticarcinogenic Medicines And Their Interaction With DNA

Study on the properties of anticarcinogenic medicine and their interaction with DNA is very significant in treatment of cancer and synthesize of new anticarcinogenic medicine. While it is an adcancing topic for people to use various modern electrochemical methods with DNA modified electrode to investigate the interaction of anticarcinogenic medicine with DNA. In this thesis, shikonin, cryptotanshinone and 5-fluorouracil (5-FU) were selected as the objects for several aspect studies: In Chaper 2, the electrochemical behavior of shikonin was investigated by cyclic voltammetry, linear sweep voltammetry, chronocoulometry and bulk electrolysis with coulometry on a glassy carbon electrode. Triple strand DNA modified electrode was preparated and used to investigate the interaction of shikonin with DNA. It was found that it is hydroquinone of shikonin to embed in DNA and interacted with guanine and adenine of DNA. In Chaper 3, the electrochemical behavior of cryptotanshinone was investigated on glassy carbon electrode (GCE) in detail, and its adsorptive behavior and redox mechanism was also discussed. The preparation and characterization of self-assembled DNA modified electrode was preliminary discussed. Furthermore, this electrode has been used to investigate the interaction of cryptotanshinone with DNA. In Chaper 4, several modern electrochemical analytical methods were used to study the electrochemical behavior of 5-FU. The voltammetric behavior of 5-FU interacting with DNA on GCE was investigated, and the kinetic parameters of 5-FU under two conditions was calculated and compared (one is in the presence of DNA and the another is in the absence of DNA), based on which it could be concluded that 5-FU really interacted with DNA.