| Objective: To study the antitumor effects and the mechanism of ABP (Agaricus blazei Murill Polysaccharides) on the Bel-7402 cells. Methods: Using MTT assay to observe the inhibitory effects of ABP on the growth of Bel-7402 cells, examine directly cytotoxinic action in vitro; Preparation the conditioned medium of ABP - activated splenic lymphocytes (ABP-SL-CM), the effects of ABP-SL-CM on the proliferation of Bel-7402 cells were evaluate d by MTT assay. Flow cytomertric analysis were used to detemine the apoptotic cells and intracellular tumor inhibitive gene p53 expression and the change of intracellular calcium levels; Electron microscope was applied to observe the morphological change; Mice bearing in vivo transplanted tumor were also used to evaluate the antitumor effects of ABP. Results: when ABP-SL-CM treated Bel-7402 cells for 48 hours in vitro, the ABP-SP-CM were found to suppress the proliferation of Bel-7402 cells and induce cell apoptosis; Flow cytomertric analysis revealed that 21.68% ( ABP-SL-CM-L) apoptotic cells were seen while in control culture (N-SL-CM) there were only few(8.14%) apoptotic cells; The expression of intracellular p53 expression had not apparently changed, while the change of intracellular calcium levels was significantly higher than that in control groups. Conclusion: ABP can inhibit the growth of liver cancer, the mechanism was related to enhancing mice splenic lymphocytes activity and inducing apoptosis, the mechanism of apoptosis has no relationship with p53 expression, but with the significantly increasing intracellular calcium levels.
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