| Objective: Adult mammalian axon regeneration is generally successful in the peripheral nervous system but is dismally poor in the central nervous system. But recent discoveries have forced a reconsideration of this accepted principle. To observe how a cut axon in the injuried rat spinal cord is induced to regenerate; the recovery of the paralyzed lower extremity after T10 spinal cord injury; if the transplanted Schwann cells can keep alive and how they function in the injuried cite. At the same time, we examined the remyelination of the axons accomplished by Schwann cells.Methods: Wistar female rats weighing 250 - 300g were used in this study. All animals were allowed free access to laboratory chow and tap water in day/night regulated quarters at 25° C. Animal care and experimental procedures complied with the Guide for the Care and Use of Laboratory Animals and were approved by the local Animal Studies Committee. Animals were randomly assigned to 3 different study groups as follows: research group C, (n=10): Autologus activated Schwann cells Transplantation; research group B(n=10): Autologus Schwann cells Transplantation; control group A(n=10):20% DMEM injection into the injuried spinal cord. Anesthesia was induced in a chamber containing 3% halothane and maintained by injection of 10% Chloral Hydrate Mit into abdominal cavity. Unilateral saphenous nerve was harvested directly (groupB) and after ligation 1 week later (groupC).or just operated out (groupA). We selected the tissue clot method to culture the... |
