Treatment Of Spinal Cord Injury With Co-graft Of Autologus Activated Schwann Cells And Umbilical Cord Mesenchymal Stem Cells

[ Objective ] To explore the activation of Schwann cells and umbilical cord mesenchymal stem cell growth and differentiation of their respective properties, analysis of the interaction between the two, and transplantation in the repair of spinal cord injury in the role and methods of evaluation of the effect of acute spinal cord injury.[Method] Through unilateral ligation Wistar rats into the saphenous nerve to activate Schwann cells in vitro and isolation, culture and purification. From pregnant rats umbilical cord was isolated and cultured mesenchymal stem cells and in vitro, purified of its growth characteristics and to the ability of nerve cells. And in vitro self-activated Schwann cells and umbilical cord mesenchymal stem cells joint training and observation of the difference between the mutual influence and role, and the umbilical cord mesenchymal stem cells into neural cells into the induction. Wistar rats 60 to IMPACTOR MODEL-Ⅱof the establishment of acute spinal cord injury T10 model. Were randomly divided into four groups, each 15: DMEM simply transplant the control group, since the activation of Schwann cells (autologus activated schwann cells, AASCs) transplantation group, the umbilical cord mesenchymal stem cell transplantation group (umbilical cord mesenchymal stem cells, UCMSCs), AASCs joint UCMSCs transplantation group. After a week in each group were randomly selected eight pairs of animals function evaluation (BBB score). After 12 weeks each of two randomly selected animals 10% BDA nerve-line tracer tagging, tagging after two weeks (after 14 weeks) the animals were killed. Remove animal spinal cord injury for 5μm rapid frozen section, a Cy3 fluorescence probe staining, fluorescence immunohistochemistry and HE staining, sections stained with microscopic image analysis software Image pro plus analysis. Using analysis of variance (ANOVA) and SNK-q for statistical analysis, comparing the group's significant difference.[Results] AASCs the separation and purification after in vitro stability-4 generation, AASCs microscope can be determined from the shape, growth is doing fine. UCMSCs AASCs co-culture and good growth. Mixed after cell transplantation group and the Schwann cell transplantation in rats hind legs sensory and motor function than the other two were significantly; BDA anterograde tracing, HE staining, fluorescence immunohistochemistry staining showed that mixed-cell transplantation group and the Schwann cell transplantation Group than simply mesenchymal stem cell transplantation group have more nerve fibers through the defect site, while the control group almost no nerve fiber regeneration through; after experimental rats hind legs sensory and motor function than the control group was, BBB score four weeks after the experimental group than the control group functional recovery Obviously, each group had a significant difference between (ANOVA test, P <0.05). BDA anterograde tracing, HE staining, fluorescence immunohistochemistry staining showed that transplantation group and the Schwann cell transplantation group with more than simple group of nerve fibers through the defect site, while the control group almost no nerve fiber regeneration through, Each group had a significant difference (p <0.05).[Conclusion] AASCs UCMSCs to each other and differentiation, can coexist. UCMSCs AASCs can induce nerve cells to differentiation, AASCs secretion can be a lot neurotrophic factor, and can significantly promote acute spinal cord injury after the regeneration and restoration of limb function; UCMSCs can contribute to acute spinal cord injury after the regeneration and restoration of limb function. AASCs joint UCMSCs transplantation in the treatment of acute spinal cord injury allows some nerve fiber regeneration.