Function Of Regulatory T Cells On Asthma Pathogenesis

Bronchial asthma is a serious chronic disease with the significant symptoms like a chronic inflammation involved by various inflammatory cells including eosinophile granulocytes, labrocytes, T lymphocytes and the other cytokinins as well.,The recent researches proved that the cytokinins such as IFN-γ produced by Th1, IL-4 generated by Th2 play important role in pathogeneses of children asthma. CD4+CD25 regulative T cells, a subgroup of T cells, which are derived from thymus and recently found, can make down regulation in the body and make immunosuppression and immune hypoergy.It has not been reported how CD4+CD25+ regulative T cells express in the bodies of the asthma children, we detect T cells in PB of the asthma children, their subgroups and secretory cytokinin in three planes. We take the measure of flow cytometry to detect cytokinin and subgroups of lymphocytes in these cells, directly observe the expression of T cells and their subgroups and cytokinin in PB of the asthma children, which is used for further studying the pathogenesis of asthma and providing a certain theoretical evidence and approach of clinic therapy of the asthma children.. Methods 1) 10 control cases.We make health examination an epidemic prevention station in Hefei city, diagnosticting 15 cases of bronchia asthma. 2) the measure of flow cytometry to detect cytokinin in the cells 3 ) the measure of flow cytometry to detect lymphocytes Ways of statisticsAll the statistics are documented in Excel and analyzed with SPSS 10.0 and each group of statistics are indicated unevenly.Each group of statistics are indicated with medians and quartiles; the difference between every two groups is verified by Mann-Whitney Test with the criteria - a=0.05 and the comparison among the active state of T cell subgroups, secretory degree of cytokinin and percent of CD4+CD25+ is chosen to be analyzed with the rank correlation of Spearman.Results 1) Finding of examining lymphocyte subgroups of CD3+T% CD4+T% CD8+T%: there is no difference between the control groups and the asthma groups by examining the number of Pin each group that is more than 0.05 averagely.2) finding of cell gene examination: CD4+T% in asthma groups is higher than that in control groups with the far difference- P=0.048 while P=0.017 in IFN-γ% between these two groups with IFN-γ% in the normal group is obviously higher than that in the abnormal one. There is no difference about IL-4 % in both groups with the figure- P=0.412.3) Comparing IL-4+IFN-γ+(both positives)% in both groups, there is nodifference between these two groups with the Mann-Whitney Test. So doIFN-y/IL-4 in both groups.4) CD4+CD25+T%: P=0.012 indicates there is a great difference betweenboth groups with the figure in the abnormal group lower than that in thecontrol one.CD4+CD25+ strongly positive %: P=0.012 shows that there is a greatdifference between two groups.CD4+T%: P=0.3 indicates that there is no difference between two groups.Lymphocyte%: P=1.16 shows that there is no difference between twogroups.Conclusions 1) Under the same condition, CD4+T cells in the body of asthma children are more easily to activate and reproduce. CD4+T cells combine with MHCII in the adaptive immunoresponse at the beginning of asthma to reinforce the interaction between T cells and antigen presenting cells and participate in signal transduction of activation signals caused by TCR discrimination antigen. 2) The expression of IFN-y in the asthma children is dramatically lower than that in the healthy children while the expression and function of IL-4 in the asthma children is relatively higher, contributes to the birth of IgE, the activation of eosinophile granulocytes and the inflammatory reaction of airways. 3 ) The percentage of CD4+CD25+regulative T cells in CD4+T may be...