Effects Of P53 Gene On Drug Resistance In Humanlung Cancer Cell Lines

Objection To investigate the effects of p53 gene on drug resistance in human lung cancer cell lines.Method 801D, a human lung cancer cell line and other four kinds of cell lines with p53 gene of different status derived from 801D were obtained. MTT assay was taken to determine the drug resistance of each cell line to chemotherapy agents(PDD,TAX,5FU,NVB,GEM). Cell cycle and apoptosis induced by antitumor drugs were detected by FACS.Results IC50 of the cell line with antisensep53 (0.26±0.09μg/ml) to PDD decreased markedly compared with 801D(0.55±0.19μg/ml, P<0.05). The cell line with wtp53 showed a smaller value of IC50(2.34±0.43 ng/ml) to TAX than 801D(8.40± 1. 50 ng/ml, P<0.05). A similar result was obtained when we deteced the drug resistance to 5FU(IC50: wtp53 2.08±0.18μg/m, 801D 4.90± 1. 12μg/ml, P <0.05). The cell line with deletion C terminal p53 has notably declined IC50 compared with 801D(P<0.05 ). By the assay of FACS, G2 phase arrest was observed when the cell line with antisensep53 was treated with PDD. TAX induced G2 phase arrest in the cell line with wtp53. A increased S phase proportion was induced by 5FU. The cell lines experienced apoptosis and necrosis when they were treated with any one agent among PDD/TAX and NVB.Conclution p53 mutation is related to drug resistance of PDD. p53 deletion connects with chemoresistance of TAX and 5FU. Neither p53 mutation nor p53 deletion is associated with drug resistance of NVB and GEM. It is helpful to choose sensitive drugs according to the p53 status of patients for enhancing the efficiency of...