Study On The Anti-shock Effect Of Shenmai And The Influence On IκBα And TLR4 Expression

The hemorrhagic shock and endotoxic shock are the commonreasons that lead the patients to death in clinical practice. Thereare many factors involved the pathological changes during the shock.A large quantity of data indicate that many signal transductionpathways are involved when the body treated with hemorrhage andendotoxin. A lot of anti-shock medicines have been used in theclinical treatment. Chinese traditional herbal medicines have manyadvantages such as low toxic, less side effects, general regulationand the source is abundant. Thereby seeking an effective naturalChinese traditional medicine is very important and practical forthe shock treatment. In this study we use "Shenmai"Injectionsolution to treat the endotoxic shock rats and "two-hit "shockrats which treated by hemorrhage and lipopolysaccharide.In order to explore the molecular mechanism of the anti-shockeffect of Shenmai and observe the influence on TLR4 and IκB mRNAexpression in endotoxic shock and "two-hit"rats spleen. Theendotoxic shock rats were divided into Control, LPS, LPS+SM andLPS+Dex group respectively. The"two-hit"rats were divided intoHS, HS+LPS, HS+LPS+DEX and HS+LPS+SM group respectively. HS+LPSinjury model of rats were induced by hemorrhagic shock(MAP 40mmHgfor 10 minutes)followed by iv administration of lipopolysaccharide(LPS,1.5mg/kg).Four hours later, the lung TNF-αcontents weremeasured by ELISA, TLR4 mRNA and IκBαmRNA expression were assayedby RT-PCR, ultrastructural changes of the type Ⅱalveolar cellwere observed by transmission electron microscope. The most results are as follows: 1. In the endotoxic shock experimental groups, compared withthe Control group, TLR4 mRNA expression in the experimental groupswere increased significantly(P<0.05), of which LPS+SM group waslower obviously than LPS group(P<0.05); IκBαmRNA expression inthe experimental groups were decreased significantly(P<0.05), ofwhich LPS+SM group was higher obviously than LPS group(P<0.05). 2. In the "two-hit "experimental groups, compared with theHS or HS+LPS group, TLR4 mRNA expression in the experimental groupswere increased significantly(P<0.05), of which HS+LPS+SM groupwas lower obviously than HS and HS+LPS group(P<0.05); IκBαmRNAexpression in the experimental groups were decreased significantly(P<0.05), of which HS+LPS+SM group was higher obviously than HSand HS+LPS group(P<0.05). 3. The lung TNF-αcontent level in HS+SM group and HS+LPS+SMgroup was obviously lower than that in HS group and HS+LPS group(P<0.05). 4."Shenmai"has a protective role on the hung tissueinflammation and injury. On the observation of lung tissue underthe transmission electron microscope, in the group treated with"Shenmai", the lung pathological injuries were alleviatedgreatly as compared to HS group and HS+LPS group. From the observation of the lung tissue and the expression of...