| Tu-Si-Zi, Semen Cuscutae,has long been used in traditional Chinese medicine as tonifying the kidney and strengthening yang with the first record in "Shengnong Bencao Jing".The seed of Cuscuta chinensis Lam. (Convulvulaceae)was accepted by Chinese Pharmacopoeia(2005). But ,the three commercial species of Tu-Si-Zi are sold in the civil markert. There were few studies performed on their identification , quality control and effective principles. This paper deals with quality study on commercial Tu-Si-Zi, Semen Cuscutae , including survey on drug resources , morphological identification ,quality analysis , and pharmacological research .Herbalogical studies showed that there were two species of Cuscuta used in ancient time , viz. big size seed (Cuscuta Japonica)an6 small size seed (C. Chinensis) repectively .Identification of 42 Tu-Si-Zi samples from the civil market indicated that the commercial species of Tu-Si-Zi were derived from C. Chinensis , C. australis and C. japonica repectively .The commercial species of Tu-Si-Zi were analyzed by TLC, HPLC, NIR, andHPCE .The results showed that there were obviously distingushed featuresamong infra-species or isotype in different regions and other confusing-used crude drugs .The characteristic features from them were obtainedthat represent the chemical difference of samplesThe systematic quality analysis of Tu-Si-Zi was made .The 40 and 27 compounds of naphtha were isolated and identified by GC-MS from C. australis and C. japonica respectively.We studied the optimal conditions and parameters for the enrichment process of flavones in C. australis with macroporous resin with the enrichment degree of flavones in C. australis as an index. Then, we found...
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