Quality Control Methods Study Of Luobuma Leave And Its Active Fraction

Luobuma (Apocynum venetum Line) was known as a kind of traditional Chinese medicine and used for treatment of hypertension, headache and blackout. Because of week quality control technique ,other plants from different sources,such as Poacynum hendersonii(Hook.f.)Woodson) and Poacynum pictum(Schrenk)Baill have been mistakenly used as Apocynum venetum Line for a long time.So, the quantificational and qualitative quality control methods of Luobuma leave and its active fraction had been estabalished and could be used as quality standard. The content of quercetin in hydrolysate of leaves had been determined by HPLC method firstly.But the result was found not to be exclusive enough.So,the UV method was established to determine the content of total flovonoids in Luobuma leave and its active fraction. Hyperin was selected as good representative consituent, which be colored by AlCl3-NaAc. Then, the HPLC method was established to determine the content of hyperin in Luobuma leave and its active fraction. All the methods of determination were accurate and presible. In this paper, total flovonoids and central active consituent-hyperin of samples from different species and areas were analysized by UV and HPLC methods respectively. Valuable conclusion had been drawn and great difference of content of total flovonoids and hyperin was found in samples of different species and areas. Exploratory researchs by chromatographic fingerprint of samples were done subsequently.Some useful harvests have been got by general TLC and microemulsion thin-layer chromatographic fingerprint. And the later shows advantage to discriminate Apocynum venetum L with its confused plants. The HPLC chromatographic fingerprint method also was established to reveal mass information from samples. Different unique peaks were found in samples from both species. And the particular section from 18 to 38min in the plants of Apocynum Linn. was found to be different from the unique section 8.9~20min in those of Poacynum Baill.. Based on those information mentioned above, Luobuma leave of different species could be identified easily. Analyzing by Computer Aided Similarity Evaluation System, the similarity of Luobuma leaves from the same aeras was 0.88~1.00, which from different species was 0.5~0.6. In a word, the content determination methods of total flovonoids and hyperin in Luobuma leave and its active fraction had been established firstly .Then three chromatographic fingerprint methods were applied on the samples from different species and areas. The experiment was a useful exploratory research.