The Screening Of New Function Genes In CNS Involved In The Obesity And Its Relative Disease

Obesity is one of the most serious disease, that terribly harm human being's physical and psychological health in the current modern society, but the effective way of curing the disease is not available until nowadays . The main reason is that we do not completely understand the mechanism of obesity, especially the molecular knowledge that lead to fatness. In the experiment, aimed at the function gene involved in the obesity and its relative disease in the center nerve system (CNS). After injected in the mice brain ventricle, we observe the diversification of the weight, the amount of feeding and drinking, apparent assimilation rate, and so on to find the gene function related to fatness. Meanwhile, carry on the animal behavior test , including Open Field Behavior, Hole-board test, Tail Flick Latency (TFL) test and Step-down test to discover the gene function on the whole animal level. In addition, during the experiment , we have mended the experiment platform to expect to obtain more objective and more accurate result . Through the experiment ,we found a gene named TUB , after whose protein expression been blocked, the mice feed more than control ,which is in according with report ,and a new gene named GPR39 whose function is unknown to us , It was found that the feeding amount of antisense group is significantly less than the control group. The rearing times of the antisense group is much more than the two control groups, and the TFL time is longer than the controls. the results of the experiments show that the gene GPR39 can regulate the animal's food intake, locomotion behavior and increase the sensitivity of pain and testified the method we used to screen function gene is viable .