The Study On Immune State Of SARS Patients In Convalescence

AIM: 1.To investigate the expressions of subsets of peripheral T lymphocyte and the relative activated molecules in healing patients with sars who left hospital. 2. To study the expression pattern' s changes of 24 repertoire TCR V β in the convalescent patients with severe acute respiratory syndrome (SARS). 3. To study the genotype of HLA-A,B and DRB1 of SARS patients and explore the association of gene alelle and SARS disease.METHODS:1.The SARS patients who left our hospital after the treatment of integration of Chinese and Western medicine were detected CD3+, CD4+, CD8+, CD8+/CD28+, CD8+/CD28-, CD3+/CD25+, CD3+/CD69+, CD3+/HLA-DR+ and CD4+/CD8+ by flow cytometry. 2. The expression of 24 repertoire TCR Vβ in peripheral blood in 76 convalescent SARS patients were detected by flow cytometer. 3. Polymerase chain reaction -sequence specific oligonucleotide robes(PCR-SSP) was used to determine the genotype of HLA-A, B and DRBl with SARS. 4. using descriptive analysis and t-test to compare the resultsRESULTS:1. In three follow-ups, the counts of CD3+ and CD3+/CD4+ wereremarkably lower than the reference (P<0.05) .The results of relativeactivated molecules appeared descend trend.2. The expression of TCR Vβ14was the highest in 24 repertoire TCR Vβ both in SARS group and in normal Controlgroup .followed by the expressions of TCR V β 5. 3 and TCR Vβ 23 ,but theexpression of Vβ 20 in normal group. There was preferential TCR Vβ expressionin SARS patients. Besides, the expressions of TCR V β 1, V β 5. 2, V β 5. 3, V β 7. 2, Vβ9, Vβ11,Vβ 13.1, Vβ 13. 2, Vβ 17, Vβ 18, Vβ 22 and V β 23 in SARS group were allsignificantly higher than that in normal group. 3. The frequencies of HLA-A34(P=0.027) ,-B60 (P=0.003) ,-B16 (P=0.004), -B58 (P=0.034), and -DRB1*12(P=0.038) in SARS patients were all different from normal control, but therewere no significant betwween SARS patients and normal controls.CONCLUSIONS: 1. Along with the time of convalescence prolonged, the effects of virus on subsets of peripheral T lymphocyte and the relative activated...