Patterns Of Cell Death Induced By Magnetic Attachment In L929 Cells

ObjectivesThe purpose of this study was to investigate in vitro the apoptosis - and necrosis - inducing potential of 261 ferrite stainless steel in 1929 cells and to e-valuate its biocompatibility . We evaluated the effect of direct contact with dental casting alloys by AnnexinV - FITC/Propidium iodide assay. Comparisons to Tilite and CoCr alloy were performed. We validated that the rate of cell death by apoptosis induced by such dental alloys could be an important indicator of their cytotoxicity degree.MethodsTest materials: Three groups (Tilite, CoCr and 261 ferrite stainless steel) of test specimens were prepared to discs, 12mm in diameter and 1mm in thick, highly polished on one face, then washed in an ultrasonic vibrobath, degreased with ethanol and autoclaved before use.Cell culture; Resuscitative L929 cells were maintained in a controlled atmosphere of 5% CO2 at 37℃. They were allowed to reach confluence before sub-culturing, only cells with a viability >95% were used in the experiments.Exposure of cells to discs; L929 fibroblasts were seeded on discs for 18h and 24h.Annexin - FITC/Propidium Iodide assay: At the culture endpoints cells were stained with AnnexinV - FITC and PI according to the manufacture's in-...