| Recent researches showed that apoptosis was found and involved in secondary cell death in the neurodegenerative diseases, Alzheimer's deasease(AD), epilepticus, cerebral ischemia, spinal cord injury and traumatic brain injury. Hower, recent evidence suggests that similar to programmed cell death or apoptosis may be contributing to the death of neurons following an ischemic insult. Extensive cerebral hemispheric infarction associated with massive brain swelling is known as malignant infarction because of the rapid clinical deterioration and mortality as high as 80%-90% unless appropriate treatment is performed. It is reported that Decompressive Craniectomy is an effective treatment. And it can reduce not only the brain inarct volume but also neurologic score affer permanent middle cerebral artery(MCA)occlusion, Early surgical decompressive craniectomy for malignant middle cerebral artery(MCA) Infarcition provides life-saving benefits; VEGF plays a significant role in regulating angiogenesis and vasculoformation during mammalian embryonic development.VEGF is a Protein that is essential for angiogenesis.Ischemic cerebral infarction associated with extensive edemand marked elevation of intracranial Pressure(ICP)may cause ischemia of neighboring brain tissue and thus leadto further infarction, how to reduce ICP and ameliated the ischemia of neighbouring brain, and eluciated the mechanisms of the treatment is the objective of experiment.Materials and methodsSprague-Dawley rats were divided randomly into middle cerebral artery occlusion(MCAO) group, Decompressive craniectomy(DC)-treated group rAd-hVEGF165 treated group, DC and rAd-hVEGF165 -treated group(uni group) ,each 8. There was no significant difference among the average values of body weight in 5 groups by ANOVA test (P>0.005).The SD-rats were anesthetized with intraperitoneal injection of 10%Chloral Hydrate(0.3ml/100g) and placed in a stereotaxic fram. The scalp was listerized with iodine and 75% alcohol and incised on the midline. An atraumatic craniectomy was performed by removing the right parietal bone posterior to bregma, 2mm lateral to the sagittal, and 2mm posterior to the coronary suture, to from a window about 4mm in diameter. An stainless needle connected with a micropump was penetrated into the right lateral ventricle at the coordinate: Ap 0.8mm, Lat 1.5mm, Dep 4.5mm in the same craniectomy window.A midline incision in the neck was performed. Afterwards, the common carotid artery(CCA) was identified. The bifurcation of the CCA , the external carotidartery(ECA), the internal carotid(ICA), the occipital and thyroid artery was ligated, which branch off the EC A, and the distal EC A itself was ligated. The Proximal ECA was clipped and a nylon o.26mm in diameter was inserted into the ECA.. nylon advanced towards the ICA to the cranium , nylon in the vessel is 18.5±0.5mm from the bifurcate of the CCA. Then fixed the nylon, sutured the skin. According to Bederson's scale, the rat' score is above l,then the model is successful.1 hours after the MCAO, We performed craniectomy by taking out a bone fiap(10mm x 8mm)in the parietal and temporal bone using a dental drill as described in detail recently. Forty-eight hours later, after Bederson's scale, the brains were removed, and then coronally sectioned into 2.5mm slices. Slices were incubated in a 1% solution of 2, 3, 5-triphenyltetrazolium chloride(TTC) at 37 ℃ and fixed by immersion in a10%buffered formalin solution about 30 min . Infaction volumes were measured by summing the unstained areas on TTC-stained brain slices.To avoid overestimation of the infarction volume, the corrected infarction volume was calculated under computer. Disaggregation of the brain tissue into singled-cell suspension and FCM analysis. First, a transverse cut was made along the inferior cerebral vein and the inferior portion often brain was discarded ,As inverted V-shaped section was then made along the medial aspect of the cortex at the edge of the infarction as best approximated by the change in tissue Pallor, The...
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