Role Of Hepatocyte Growth Factor In Diabetic Cardiomyopathy

Objective: To explore the role of hepatocyte growth factor in diabeticcardiomyopathy, to provide the theoretical foundation for diagnose and therapyearly in diabetic cardiomyopathy. Methods: Seventy Wistar rats were divided randomly into two groups.There were thirty in normal control group(NC) and forty in diabetes mellitusgroup(DM). The subjects in diabetes mellitus group were injectedintraperitoneally with low dose streptozotocin(STZ, 30mg/kg) after high caloriediet given for two months, while the ones in normal control group were injectedintraperitoneally with the similar dose of citric acid buffer. Before injection, onemonth, two months, three months and six months after injection, there were sixrats in both groups respectively killed. The blood samples were obtained tomeasure the levels of HGF, , INS, Cho,Tri, et al. The heart samples were used toanalyse morphology. We compare these data between the two groups and amongthe different stages with factorial designed analysis of variance to reveal the roleof HGF in the pathogenesy of diabetic cardiomyopathy. Results: 1.There was a difference in the level of fast blood glucosebetween NC and DM after high calorie diet given for two months(P<0.01). Thedifference was significant after injected intraperitoneally with STZ and the levelof FBG was increasing with the progression of DM, then at the level of16.5-16.8mmol/L stably. In addition, there were a significant difference in thelevels of triglyceride and cholesterol between the two groups at everystages(P<0.01). These changes are similar to type 2 diabetes mellitus. 2.Compared with NC, there were significant differences in the level of INSand insulin resistance index after high calorie diet given for two months(P<0.01). The differences between the two groups had existed for the wholecourse of the experiment. The phenomena are also similar to type 2 diabetesmellitus. 3. Compared with NC, the changes of myocardium ultrastructure wereobvious,myocardium mitochondria was edematous,myocardium endothelialground membrane was thicker at month three after injected STZ (P<0.01).Myocardium cell was bigger than NC after high carorie diet given for twomonths, therefore, these changes were more significant at month six. 4.The level of HGF of DM was much higher than NC. It increasedgradually after injected with STZ and was at the highest level at two months.Then it decreased gradually. Conclusion: 1.High blood insulin and insulin resistance were made in therats after high calorie diet given for two months. Then the rats were given lowdose STZ to destory some beta cells. All of these were similar to the process oftype 2 diabetes mellitus. 2. Observed pathologically, myocardium cell was bigger than NC after highcalorie diet given for two months. Myocardium ground membrane was thicker atmonth three after injected STZ,these changes were obvious at the end ofexperiment, All of these proved diabetic cardiomyopathy wase serious. 3.The level of HGF of diabetes mellitus group was much higher thannormal control. Furthermore, the levels of serum HGF increased gradually at thebeginning of DN and decreased gradually after three months. According thesewe can conclude that HGF may play a protective role in the pathogenesy of DN.