Experimental Study Of Abenovirus Mediated Mouse Flt3-L Gene Therapy To G422 Glioblastoma

Objective: Our study is to investigate the therapeutical effect of mouse Flt3-L gene to G422glioblastoma.Methods: Our study is to adopt mouse Flt3-L gene mediated by adenovirus (Ad-Flt3-L) to treat G422glioblastoma of mouse. The effect of transfection to G422 glioblastoma cells was observed throughgreen fluorescence protein gene mediated by adenovirus (Ad-GFP). The Flt3-L gene in recombinantadenovirus was detected through protein Flt3-L by RT-PCR, Western-blot. Proliferation of G422 cellsafter transfection was measured through 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromidetest(MTT).Results: When multiplicity of infection(MOI) is 100, the transfection efficiency was more than 90percents in 36 hours after transfection to G422 glioblastoma cells through green fluorescence proteingene mediated by adenovirus (Ad-GFP). When these cells was detected with reverse transcriptionPCR(RT-PCR) after split ,a high bright strap about 620 bp was found between 600bp and 700bpstandard DNA in Ad-Flt3-L group, while this phenomenon didn't occur in control group. ProteinFlt3-L of high level was found in supernatant fluid through Western-blot test after transfection. G422glioblastoma cells can grow normally after thransfection with Ad-Flt3-L by MTT test.Conclusion: This confirmed that gene Flt3-L carried by adenovirus had no influence to G422 cell'snormal differentiation and proliferation.