Chemotherapy Research Of G422 Glioblastoma In Mice Using Doxorubicin-loaded Nanoparticles

Objective: To evaluate the therapeutic potential of doxorubicin-loaded nanoparticles in vivo using an animal model created by implantation of G422 glioblastoma in mice brains.Methods: We manufactured doxorubicin-loaded nanoparticles and blank nanoparticles by anionic polymerization. We implanted G422 glioblastoma cells in mice white matters. Mice having received implantation were randomized into 6 groups, which received varied doses of doxorubicin-loaded nanoparticles or doxorubicin in saline or no treatment respectively. The preparations above were injected i.v. into the tail veins on days 2, 5 and 8. We randomly selected two mice, which received FCM and H.E. Stain respectively, from the groups treated with 3×2.5mg/kg doxorubicin-loaded nanoparticles, doxorubicin in saline and no treatment on day 10 respectively.Results: Mice treated with doxorubicin-loaded nanoparticles in highest dose had significantly higher survival times compared with the group of doxorubicin in saline. The therapeutic effects of varied doxorubicin-loaded nanoparticles correlated with the dosages of doxorubicin-loaded nanoparticles. Histologic pathological examination of brains confirmed nontreated mice developed a maximal tumor size, while mice treated with doxorubicin-loaded nanoparticle in highest dosage developed a minimal tumor size. Mouse treated with doxorubicin in saline had diffusely invasive tumor growth; in contrast, mouse treated with doxorubicin-loaded nanoparticle in highest dosage had solid tumor growth with clear margin. FCM showed nontreated tumor had highest apoptosis percentage, while tumor treated with high dosage of...