Expression Of PTI-1 In Prostate Cancer And The Study Of Its Function

Prostate cancer(Pca) is the second leading cause of death of men in Europe and North America. Prostate cancer is characterized with the latency and high incidence. In China, 25 percent of men over 70 suffer from prostate cancer, but only a few can become the patients with clinical symptoms. Until now, serum PSA level is the most effective way to diagnose prostate cancer in early stage. But serum PSA level is affected by many factors, we call for a new tumor marker of prostate cancer which has more specificity in diagnosing of prostate cancer.Prostate tumor inducing gene 1 (PTI-1) cloned from a human prostatic carcinoma cell line, LNCaP, was indicated as a dominant- acting tumor inducing gene. By using cotransfection nude mouse tumor assay and differential RNA display (DD) technology, Fisher PB initially detected this putative oncogene in nude mouse tumor derived CREF-Trans6:4NMT cells (CREF-Trans 6 cells transfected with LNCaP DNA), whereas it was not expressed in parental CREF-Trans 6 cells. The 2106 bp full- length PTI-1 cDNA consists of three regions: a 5' unique 620 bp region (1-620 bp), an open reading frame from 621bp to 1814bp encoding a protein of 398 amino acids and a 3'UTR (1818—2106 bp). PTI-1 has oncogenic properties and its transcripts were expressed in various human tumor cell lines, prostate cancer...