| Developments in transgenic technology have greatly enhanced our ability to understand the functions of various genes and their relevant human diseases. Whereas, inducible gene expression systems have typically encountered limitations, such as plethoric effects of the inducers, basal leakiness, toxicity of inducing agents and low levels of gene expression.The tetracycline-regulated gene expression system developed by Gossen and Bujard is a most frequently cited and a powerful genetic tool that permits the expression of any gene construct introduced into either cultured cells or transgenic animals to be precisely controlled. It has been applied in numerous areas of bioscience. This system involves two components, a regulatory component based on the prokaryotic tetracycline repressor (TetR) and a response plasmid that expresses the gene of interest under control of the tetracycline-response element.To achieve the regulated gene expression on the Hodgkin Lymphoma derived cell line-L428, we review the T-REx system methodology, describe how to create, isolate and characterize the tetR expressing cell lines which express a gene of interest under the control of tetracycline.By employing the western blot and immunohistochemistry techniques, we observed a distinct difference on the expression of both the positive control and dominant report genes between the induced and their non-induced counterparts in L428TR1 cell line. It indicated that the T-REx system isavailable on Hodgkin Disease cell line and excellently demonstrated that our isolated L428TR1 cell line is capable of supporting the inducible expression of the gene of interest upon the tetracycline. More importantly, it would greatly facilitate the transgene research in Hodgkin Lymphoma which would benefit us profoundly to further understand more functions of many genes on the Hodgkin Disease under the control of their expression and ultimately may show some new lights in the whole field of Lymphoma.
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