The Expression Of CLIC1 And LyGDI In The Colorectal Cancer And Their Clinical Significance

Objective:Colorectal cancer is a common malignant cancer. Its incidence and mortality rank 3 in all cancers. In the recent year, the incidence has become higher and higher in our country. There is no obvious clinical symptom in colorectal cancer patient when it is at early stage. When the patients have obvious symptom, the colorectal cancer is at the advance stage, so the prognosis of the colorectal cancer is very low. The mechanism of the colorectal cancer is very complex. Many researches reveal that some oncogenes are activated and some tumor suppressor genes are inactivated.Finding out these genes can help diagnosis colorectal cancer at early stage, and this will improve the treatment effect of surgical resection, and the incidence and mortality of colorectal cancer will become lower. Our research is detecting the differences in the expression lever of CLIC1 in adjacent tissue, adenoma tissue and colorectal cancer tissue, and also the differences in the expression lever of LyGDI in adjacent tissue, adenoma and colorectal cancer. And analyze the relation of their expression lever in colorectal cancer and the clinical pathological factor of the colorectal cancer and the survival rates of the colorectal patients.Methods1. Detect the expression of LyGDI in adjacent tissue, adenoma tissue and colorectal cancer tissue, and compare the differences of the expression lever of LyGDI.2. Construct the tissue micro-array, and explore the differences of the expression lever of CLIC1 and LyGDI in 150 cases of adjacent tissue, 52 cases of adenoma and 187 cases of colorectal cancer. And analyze the relation among the expression level of CLIC1 and the clinical pathological factor and the survival rates of the colorectal cancer patients, and also analyze the relation among the expression level of LyGDI and the clinical pathological factor and the survival rates of the colorectal cancer patients. Results:1. The expression level of LyGDI in the colorectal cancer is higher than that in the adjacent tissue by western blot technology, and the difference is statistically significant.2. The expression of CLIC1 in the adjacent tissue, adenoma and colorectal cancer gradually increases in order by immunohistochemistry based tissue micro-array, the difference is Statistically significant. The expression of CLIC1 is related with TNM staging. The higher the TNM staging, the higher the expression of CLIC1. The survival rate in the CLIC1 positive staining group is lower than that in the CLIC1 negative staining group. But the expression of CLIC1 is not related with the gender, age, tumor size, tumor differentiation and the location of the carcinoma.The expression of LyGDI in the colorectal cancer is higher than that in the adjacent and adenoma tissue by immunohistochemistry based tissue micro-array. The difference is Statistically significant. The expression of LyGDI is related with TNM staging. The higher the TNM staging, the higher the expression of LyGDI. The expression of LyGDI is related with tumor differentiation staging. The higher the tumor differentiation staging, the higher the expression of LyGDI. The expression of LyGDI is not related with the gender, age, tumor size and the location of the carcinoma. The expression of LyGDI is also not related with the survival rate of the patient. There is no significant difference between the LyGDI positive staining groupand the the LyGDI negative staining group.Conclusion: The expression of CLIC1 is in relation to the oncogenesis, development of colorectal cancer, and also the survival rate of the patient. And the expression of LyGDI is in relation to the oncogenesis, development of colorectal cancer, but not related with the survival rate of the patient. CLIC1 and LyGDI are potential tumor biomarkers for colorectal cancer.