Application Of Dendrimer DNA Hybridization In Clinical Diagnosis

â… . Detection of Human Papillomavirus Genotypes in GenitalCondyloma Acuminata Using Dendrimer DNA HybridizationHuman papillomaviruses(HPVs) are epithelium-tropic viruses associated with several cutaneous,epithelial and mucosal lesions. Until now,more than 80 different genotypes of HPV have been identified. In this study, HPV 6 and 11 which are frequently associated with benign lesions have been detected in genital condyloma acuminata. To explore a new detection method with high sensitivity and high specificity, 50 tissue samples of condyloma acuminata obtained from surgically excised specimens and 15 normal serum samples have been detected by DDH and PCR methods simultaneously. In these 50 cases with genital condyloma acuminata, using DDH method, HPV DNA was positive for 20(HPV 6), 24(HPV 11) and 3(HPV 6/11), respectively, and the total positive percentage with DDH was 94%, while 21(HPV 6), 24(HPV 11), 3(HPV 6/11) and 96% was positive using PCR method. In 15 normal serum samples, the false positive frequency of HPV DNA was 0/15(0%) and 1/15(6.67%) by using DDH and PCR method respectively. In this study, we quantified the sensitivity of HPV positive cases, DNA detection by DDH method. The lowest concentration of positive cases, DNA which could be detected was 97.28pg/ml. The results showed that DDH is sensitive, highly specific and low cost, and also is suitable for grass-roots, small or middle-sized hospitals.