The Changes Of T-Lymphocytes In Rats By Traumatic Hemorrhagic Shock And Effects Of Dexamethasone On This Event

English AbstractThe Changes of T-Lymphocytes in Rats by Traumatic-HemorrhagicShock and Effects of Dexamethasone on This Event Objective To investigate changes of T-lymphocytes in rats bytraumatic-hemorrhagic shock and effects of dexamethasone(DXM)on itsfunction. Methods With the model of traumatic-hemorrhagic shockestablished,we randomly divided rats into three groups: normal controlgroup; traumatic-hemorrhagic shock group and dexamethasone-treatedgroup of traumatic-hemorrhagic shock. TNF-αin plasma of rats in everygroup were measured at 1 h, 2 h, 3 h , 6 h and 12 h . In every group and atdifferent time,rats were sacrificed and the pathological changes of spleenlymphocyte and lung were observed by light microscopy. Meanwhile, at 2h and 6 h, the pathological changes of spleen lymphocyte were observedby transmission electron microscopy. Using immunohistochemistrytechnology, IL-2 and IL-4 in spleen were marked at every groups andtimes. Results After traumatic-hemorrhagic shock, the expression ofTNF- α increased evidently and pear levers were at 6h. Spleniccorpuscle hyperplasia at first and atrophy later were observed by lightmicroscopy. Under transmission electron microscopy, hyperplasia andactivation of T-lymphocytes were observed during splenic corpuscle 3hyperplasia period; necrosis of T-lymphocytes were observed duringsplenic corpuscle atrophy period. IL-2 was expressed byimmunohistochemistry mark during splenic corpuscle hyperplasia periodand IL-4 was expressed by immunohistochemistry mark during spleniccorpuscle atrophy period. The administration of DXM inhibited theexpression of TNF-α and alleviated the pulmonary and spleen damage.Splenic corpuscle hyperplasia remained and the result ofimmunohistochemistry mark has not changed significantly. Conclusion Firstly, T-lymphocytes were activated intraumatic-hemorrhagic shock rats, later, TH2 shift happened and T-lymphocytes function failure finally. DXM inhibited inflammatoryresponses, alleviated organ damage, preserved function ofT-lymphocytes .