The Distinctive Change Of Lipid Peroxidation In Lung Cancer And Atheromatous Disease

Objective:To investigate distinctive changes of lipid peroxidat ion (LPO) in patients with atherosis (AS) or lung cancer. To explore the correlation of different LPO in lung cancer or in atherosis. To explore the new methods and theroies in theraping cancer and atherosis on clinical. Materials and Methods:Patients with lung cancer or atherosis with dibetes mellitus diagnosticed were experimental groups. These were divided into two groups according to different cohort of patients. 20 healthy volunteers(university students) were normal control. Three experimental groups: (l) lung cancer group (n=29), (2) atherosis group (n=20), (3) healthy control group (n=20). Blood from every objects was collected in tubes added othylonedletmlno totraontic acid (BDTA) (torminnl concentration, 0. 05mmol/l), and was contrifuged at 3000r/min for 15min. Plasm was removed and stored at 0ç™± in refrigerator. Low density lipoprotein (LDL) and high density lipoprotein (HDL) were isolated from plasm by means of ultracentrifugation . LPO was detected by means of yaji in LDL or in plasm. Super oxidized dismutase (SOD) was measured by means of the radio immunoassay (IUA). Peak positions and rough concentration of.' LPO were shown by thin layer chromatography.In the course of preparing LPO, added antioxdiant and observed the creation of LPO by thin layer chromatography. Results:(1) In lung cancer patients ,LPO levels were singnificantly higher than in controls(p<0.05) (lung cancer, 9. 2+/-5. 1;control, 5. 2+/-4. 3). In atherosis patients, LPO levels were significantly higher than in controls (p<0.01) (atherosis,11. 9+/-4. 0;control, 4. 6+/-2.75). Serum level of LPO didn't differ among these patient groups. (2)The Jipid constituents were analyzed by thin layer chromatography. Distribution of LPO differs among these groups. In lung cancer patients,LPO spots located between phospholipid and free fatty acid . In atherosis patients,LPO spots located between triglyceride and free fatty acid. But, in control, LPO spots wore't apparent. (3) Patients with Atherosis or lung cancer, SOD levels were significantly higher than in controls (control, 328. 7+/H40. 53; lung cancer, 736. 7+/-366.9; atherosis, 826. 6+/-366. 9). No significant clifferanco in SOD levels was observed among those patient groups. (4)In the course of preparing LPO, thin layer chromatography showed that antioxidant could eliminate LPO spots . Conclusion: (1) LPO levels in cancer or atherosis were significantly higher than in control, which implicate that LPO was close correlation with lung cancer or atherosis ;(2)Shown by thin layer chromatography,migration velocity of LPO particles obtained from serum in patients with lung cancer was significantly slower than in atherosis, which implicate that LPO molecular weight in patients with lung cancer may be larger than in atherosis;(3)Differrent structure of LPO suggest that there were different mechanisms in their pathological process during the development of cancer or atherosis; (4)Antioxidant may effectively inhibit an increase in LPO, which suggest that antioxidant may be an effective medication in theraping cancer and atherosis.