BRCA1 Expression In Primary Epithelial Ovarian Cancer And The Relationship Of Prognosis, Clinical And Pathological Features

Objective: To detect the expression of BRCA1 protein in primary epithelial ovarian cancer, and to investigate the influence of BRCA1 expression on the prognosis, clinical and pathological parameter.Method: 81 women with primary epithelial ovarian cancer were studied. The expression of BRCAl was determined by immunohistochemical assay in which the C-terminal monoclonal antibody (Ab-3) was used. The relation of BRCAl expression and clinical pathological parameter was estimated by correlation analysis. Kaplan-Meier and Cox proportional regression survival analyses were used to compare normal and abnormal expression of BRCAl.Result: 44 (54.3%) of 81 specimens showed normal staining of BRCAl. 34 (42.0%) specimens were mislocated in cytoplasm, 3 (3.7%) specimens were absent in both nuclear and cytoplasm. In 2 of 13 normal ovarian epitheliums, BRCAl protein was detected in nuclear (15.4%). All of 12 benign ovarian tumor showed normal BRCAl staining. BRCAl has no relationship with clinical pathological parameter (P>0.05) . Kaplan-Meier showed that there was no correlation between BRCAl expression and survival. In multivariate analysis using the Cox regression model, BRCAl expression was not an independent prognosis indicator for disease free survival in primary epithelial ovarian cancer.Conclusion: In primary epithelial ovarian cancer, expression of BRCAl wasabnormal, such as decrease or absent in nuclear and mislocated in cytoplasm. BRCA1 expression was not correlated with survival and prognosis.