Experimental Studies Of Expression Of PDGF-BB/TGF-β1 After Focal Cerebral Ischemia/Reperfusion In Rats

Purpose The study aims at the function of platelet-derived growth factor BB (PDGF-BB) and the relation of PDGF-BB and TGF- ?1 in brain ischemia/reperfusion.Method Remove brain tissues from fatal cases with cerebral infarct and cases with non-cerebrovascular diseases. Rat model of ischemic reperfusion of cerebral middle artery was established. Rats were randomly divided into sham operation group, ischemia/reperfusion group, medicine treated group and TTC staining ischemia/reperfusion group. The ischemia/reperfusion group is divided into six subgroups according to reperfusion time difference such as Oh> 6h 12h. 24h. 3d and Id. MACO2h/R24h group was treated with Nimodipine. Using immunohistochemical staining method and color image analysis, the expression of PDGF-BB and TGF- ?1 in cerebral hippocampi were measured. The infarct volume was measured by TTC staining.Results Compared with control group, the expression of PDGF-BB and TGF- P 1 in infarct group in brain tissues from fatal cases increase. The ischemia volume (IV) in MCAO2h group is the smallest and the IV in MCAO2h/R3d group is the biggest. The IV in medicine treated group is smaller than the corresponding group without medicine. Expression of PDGF-BB in MCAO2h group remains the same as control group and increases after reperfusion with the peak value in 7d group. Expression of PDGF-BB in medicine treated group is higher than the expression in the group without medicine. Expression of TGF- P 1 decreases in MCAO2h group and increases after reperfusion with the climax in 48h until 7d. Expression of TGF- P 1 in medicine treated group is higher than the expression in the group without medicine.Conclusions Ischemia and reperfusion can induce expression of PDGF-BB and TGF-P 1. The two factors can protect and regulate themselves and promote blood vessels proliferation surrounding ischemic zone and help damage recovery. TGF- P 1 acts as a neuroprotection factor by the way of upregulation of PDGF-BB. Nimodipine can relieve cerebral tissue damage and increase expression of the two proteins and protect cells from damage.