| Objectives: To study the survival, growth and function restoration of heterotopic autotransplanted overy in rats. Methods: Adult female SD rats were used in this study. They were randomly divided into 3 groups: group A-Normal rats. Group B-Oophorectomize rats. Group C-Transplanted rats. In the transplanted group, ovaries were autotransplanted heterotopically under the uterine capsule and subcutaneous tissue in neck .On day 2, 4, 7, 14, 28, 56 after transplantation, the grafts were removed and underwent histological examination. Serum estrodiol and progesterone levels were determined. Results: It can be divided three periods according the HE staining. On the day 2 to 7 of transplantation, there were few follicles in different stages and most of them were primordial and primary follicles, there were some blood cells and white cells in the graft, the necrotic area account for 50%. On the day 14, there were many follicles in different developing stages, corpora luteas and interstitial glands in the graft, the necrotic area were declined. On the day 28 and later, a lot of follicles in different developing stages, corpora luteas and interstitial glands can be found. Necrosis disappeared. The uterine growth in the transplanted group was similar in the normal group . According to the change of vaginal drop cells, the estrus cycle emerged 9 days after transplantation. The results of serum estrodiol and progesterone showed that transplanted ovary restored secreting function. Conclusions: This study indicates that bilaterally heterotopic autotransplanted ovaries can be survival, develop and restore the function of secreting sex hormone.This study provided experimental basis for possible clinical ovarian tissue transplantation for the treatment of perimenopausal syndrome.
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