Expression And Significance Of Protein Kinase C-α,-βⅡ And-δ In Lung Cancers

Purpose: Protein kinase C (PKC) is known to play an important role in signal transduction pathways.PKC is a family of serine/threonine, and has been implicated in a variety of cellular functions including proliferation, differentiation, apoptosis, gene expression, membrance transmission and signal transduction.Now,the study concerning function of PKC isoforms in neoplasm initiation,promotion and metastasis has been focused.In the past the majority of study in relation to this focused on bladder cancer,prostate cancer,colon cancer,breast cancer,the combined study on expression and significance of PKC-α,-βⅡ,-δ in lung cancer has almost not reported .To investigate the expression of PKC-α,-βⅡ,-δin lung cancer and relationship among them will not only benefit understandering on lung neoplasm mechanism,but also provide an effective way for diagnosis and anti-tumor treatment of lung cancer. Method: The expression of PKC-α,-βⅡand -δ was immunhistochemically detected in 84 cases of lung cancer and their lymphatic metastastic tumors in order to analyze the different expression of PKC-α,-βⅡand –δamong different tissue types and differentiation of lung cancer;the difference of PKC-α,-βⅡand -δ between the primary lung cancer and their lymphatic metastastic tumors.Furthermore,we attempt to infer the connection between the signal transduction factor and lung carcinoma. Result: 1.The expression percentage of PKC-α in adenocarcinoma, which is 46 .9%, is lower than small cell lung cancer,large cell cancer, squamous cell carcinoma ,bronchiolo-alveolar carcinoma, which are 58.3%,66.7%,75.0% and 80.0% respectively. But there has no significant difference. In non-small cell lung cancer, the expression of PKC-αin their lymphatic metastastic tumors was much higher than that in primary tumors, the expression percentage of PKC-αare 83.3%,62.6%, and has significant difference(P<0.01). 2.The expression of PKC-βⅡhas significantly difference among different tissue types of lung cancer(P<0.05), The expression percentage of PKC-βⅡin squamous cell carcinoma,adenocarcinoma,bronchiolo-alveolar carcinoma,large cell cancer and small cell lung cancer are 78.1%,75.0%,70.0%,66.7%,41.7%, through comparison there was significant difference between adenocarcinoma and small cell lung cancer(P<0.05). In non-small cell lung cancer,the expression of PKC-βⅡin primary tumors was much higher than that in their lymphatic metastastic tumors, the expression percentage of PKC-βⅡ are 75.0%,52.7%,and has significant difference(P<0.01). The expression of PKC-βⅡ has distinctive difference between non-small cell lung cancer and small cell lung cancer(P<0.05). 3.The expression percentage of PKC-δin squamous cell carcinoma,adenocarcinoma,bronchiolo-alveolar carcinoma,large cell cancer and small cell lung cancer are 70.8%,62.5%,80.0%,83.3% and 41.7%. The expression percentage of PKC-δamong non-small cell lung cancers has no significant difference, but distinctive difference between non-small cell lung cancer and small cell lung cancer(P<0.05). In non-small cell lung cancer, the expression percentage of PKC-δ in primary tumors and their lymphatic metastastic tumors are 63.9%,80.5%, and between them has significant difference(P<0.01). Conclusion: 1.The expression of PKC-α,-βⅡ and -δ has significant difference among different tissue types of lung cancer in primary tumors and their lymphatic metastasic tumors.It suggested that PKC-α,PKC-βⅡ and PKC-δ may play important roles in invasion and metastasis of lung neoplasm.2.The expression of PKC-βⅡ and PKC-δ in small cell lung cancer is lower than in non- small cell lung cancer .It suggested that the expression of PKC-βⅡ and PKC-δ is correlated with tumor cell types and tissue origin of tumor cells.