Experimental Study Of BFGF Combined Partially Demineralized Allogenic Bone In Mandibular Defects

Objective:To explore the application of the basic fibroblast growth factor (bFGF) combined partially demineralized allogenic bone in mandibular defects, and to study the effects of bFGF in bone formation as well as the selection of its carrier.Methods:Bilateral mandibular defects were created in twenty-one adult rabbits . According to completely randomized principle, one defect was grafted with bFGF combined partially demineralized allogenic bone ,as experiment group. The other was repaired with partially demineralized allogenic bone alone, as control group. Animals were sacrified at 3,6,12 weeks after postoperation. The mandible specimens were assessed by gross inspection ,X-ray photograph, light macroscopy and scanning electronic microscope examination. Under lane-Sandhu's X-ray and histology grade standard, the results of X-ray photograph and histology slice investigation were quantified and then analyzed with statistical methods.Results: The first stage recoveries of operation incisions were obtained in both groups and there were no excluded reaction happened. In different stage after operation, there were more new formed blood vessels, fibroblast and fibrotissue and osteoid than control groups. Statistic analysis on the data of X-ray photographs and histology slices showed a significant difference between the two groups (p<0.01). The scanning electronic microscope examinations showed that in experimental groups , plenty of osteogenesis were discovered in bone defects but in control groups there were fibrotissues and little osteogenesis.Conclusion: bFGF can promote the osteogenesis in bone defects in partially demineralized allogenic bone transplantation. Partially demineralized allogenic bone can be as a carrier of bFGF and applied in mandibular defects.