The Effect Of Interleukin-1β On Early Mouse Embryo Culture In Vitro

[Objective] To study the effects of IL-1 β on two-cell mouse embryos cultured in vitro.[Methods] Female ICR mice were injected by PMSG-HCG and killed 44 hours after HCG injection. Then, two-cell embryos were collected and cultured in vitro randomly in five treatments: 1)Control: M16 medium with no IL-1 β ;2)Treatments:4 groups, M16 media with 0. 1, 1, 10, lOOng/ml IL-1β respectively . Blastocyst rates and hatched blastocyst rates were counted and calculated after 96 hour cultured.[ Results 3 652 two-cell embryos were collected and cultured for up to 96 hours.Results:(1) at 72h point, blastocyst rates were 74. 1%, 71.5%, 70%, 69% respectively for IL-1 3 at concentrations of 0. 1,1,10, lOOng/ml. The rates were significantly higher than that(43.9%) of the control (P<0.05); but there was not significant(P>.05) differention among the four rates of the treatments. (2)at 96h point, hatched blastocyst rates were 61. 1%, 56. 2%, 58. 5%, 53. 5% respectively for IL-1 β at the concentrations of 0.1,1,10, 100ng/ml;the former three rates were significantly higher than that (34.1%) of the control (P<0. 05); but the last one was not significantly (P>.05) higher than that of the control;and there was not significant(P>0. 05) differention among the four rates of the treatments.[Conclusion] Among our study range of the concentrations: IL-1β could (1) increase blastocyst rates of two-cell mouse embryos cultured in vitro. (2)improve hatched blastocyst rates of the embryos within the concentrations of 0.Ing/ml ?lOng/ml. (3)has no toxic effect on development of the embryos in vitro.