The Primary Study On Space-time Change And Molecular Mechanism Of Vasculogenic Mimicry In Mice Transplant Molenomas

Objective: To study the effect of CD31, MMP2, MMP9, CathepsinD , HMB45 on Vasculogenic mimicry in the melanoma transplant tumour. Methods: 1.Set up animal model The mouse melanoma transplant tumor model was made by Subcutaneous injecting in the coded mouse with B16 melanoma cell suspending liquid. When tumor appears, we choose one mouse each day randomly according to random number chart and kill it. 2. Staining Fix and embed the tumor specimens normally. HE stain, immunohistochemistry stain and CD31-PAS double stain. 3. Mothods of counting Counting the Vasculogenic mimicry and endothelial vessel under each high field in centre and outside area. Counting the positive cell around the Vasculogenic mimicry and far away from Vasculogenic mimicry per 100 tumor cell; Counting positive cell around the endothelial-depending vessel and far away from the endothelial-depending vessel per 100 tumor cell. 4. Data and picture managing Using computer software to calculate the necrosis ratio; Using SPSS 10. 0 statistic software to deal with data and make 0. 05 the boundary of statistical significance. Result: There are PAS-positive pattened vessels in the tumor tissue at the early phase of melanoma and erythrocytes inside the vessel. At the same time, some tumor cell mimic endothelial cell and lined inside PAS-positive vessel and form endothelial-like vessel. This is Vasculogenic mimicry. This structure decrease gradually in center and outside area and the density of the two area has no significant difference .The endothelial vessel also decrease gradually in center and outside area and the density of the two area hasno significant difference. There is negative correlation between the vasculogenic mimicry density and tumor necrosis ratio (r=-0. 978, p<0. 05); there is also negative correlation between the endothelial vessel density and tumor necrosis ratio(r=-0. 230, p<0. 05). The number of CD31 , HMB45 ,MMP2 positive cell around vasculogenic mimicry is more than that of far away vasculogenic mimicry . The number of CathepsinD,MMP9 positive cell around vasculogenic mimicry have no significant difference with that of far away from vasculogenic mimicry . The ratio of positive cell around endothelial vessel has no significant difference with that of far away from endothelial vessel. The number of MMP9 positive cell around vasculogenic mimicry is more than that of around endothelial vessel . There are correlation between vasculogenic mimicry density and the number of CD31 , MMP2 positive cells around vasculogenic mimicry . There are no correlation between vasculogenic mimicry density and the number of HMB45x MMP9 positive cells around vasculogenic mimicry. The density of endothelial vessel has correlation with the number of CD31 positive cell around endothelial vessel. The density of endothelial vessel have no correlation with the number of MMP2, MMP9, CathepsinD positive cell around endothelial vessel .Conclusion: The vasculogenic mimicry is one sort of special blood-providing pattern and its appearance makes the tumor adapt the microenvironment more freely. This structure usually co-exist with endothelial vessel in some tumor . The vasculogenic mimicry decreases gradually with tumor development and the density of vasculogenic mimicry between center and outside area have no significant difference .The endothelial vessel also decrease gradually with tumor development and the density of endothelial vessel between center andoutside area have no significant difference .The vasculogenic mimicry is more important to the tumor blood - providing than endothelial vessel in this tumor. The CD3, Cathepsin D MMP2 MMP9,, HMB45 all take part in the development of vasculogenic mimicry and CD3K MMP2 play key role in this process .