| The incidence of the intestinal adhension after abdominal operation is as high as 90%, so the intestinal adhesion is still one of the more common complications although with advancement in science and improvement in surgical operation. One study has showed that 1.2-3.6% of the patients who receive abdominal operation will be reoperated just because of intestinal adhesion. For more severe case, mesenteriopexy in needed. This is a very big personal and social problem. Therefore, how to prevent iintestinal adhesion and obstruction after abdominal operation is one of the question which most surgeon pay attention to. To prevent the postoperative peritoneal adhesion, we must understand the mechanism of the peritoneal adhesion firstly. Under normal conditions, the release Of fibrinogenase of the peritoneal mesothelium cells and fibrinolysis is kept balance, but the balance may be interrrupted by the mechanical incision, tissue ischemia, exogenous material implantation and peritonitis, which makes the release of fibrinogenase increased and the activity of plasminogen activator decreased, resulting in lots of fibrin deposition and organization and formation of fibrous adhesion eventually. Therefore,the main measurement in prevention of the postoperative peritoneal adhesion is to reduce mechanical iincision by careful operation; to prevent tissue ischenmia by maintaining well blood supply; to diminish infammatory reaction by some medcine. The researcher had developed many supplementary measurements to prevent the postoperative peritoneal adhesion. For example, to prevent fibrin deposition by the heparin to promote fibrinolysis by rt-Pa, to restrain the proliferation of fibroblast by chitoscan, but the measurements are not very effective for some reasons. It is verified that the effective contact of serious coat is necessary to form adhesion under the pathological state, many researchers had tried to utilize the llocal isolation materials (including membranous or liquid) to prevent the postoperative peritorial adhesion, but the methods can not be applied effectively, because the uncontrollable retain in the peritoneal cavity and the toxic and side-effect of the material itself.With the development of bioogical medcine, synthesis of biodegradable macromolecule material and it's application hasbecome one of the hot-spots, which provide the developing direction in using the local isolation material to prevent the peritoreal adhesion.The material of preventing postoperative peritoneal adhension should have the specific properties: 1. very well biocompatibility. 2. no toxic and side-effect. 3. completely degraded in the body with the Changchun Application Chemical Research Institute some kinds of the biodegradable macromolecule materials with the properties mentioned above. First, the intestinal adheaion model in rats was established on the basis of this, the experiment was performed with different kinds of macromolecule materials and found that the mosteffective material is Fe3+ modify sodium carboxymethyl cellulose; the most effective density is 3%; the most ettective dose is45mg/Kg.Based on the premior job, we continue to design and do the following experiments: Objective To study the mechanism of Fe3+-CMCpreventingpostoperativeperitonealadhesion.Methods1.Measuring the variation of tPA,uPA to evaluate the effectiveness of Fe3+-CMC preventing postoperative inflammatory response;2.To ebasluate the efficacy Fe3+-CMC preventing postoperative adhesion by eye and microscopic oberservation, and observing the histologicalchangingofadhesivetissue.3.Measuring the expression ofTGF-β,FGF of adhesion tissue, then observing the effectiveness of Fe3+-CMC.4.Obersving the fundational varation of all kinds of cells in adhesive tissue by transmission electron microscopy.Results:1.The amount of (in the serum of experimental group is less than that of control group(p<0.01).2.The adhesion extent of experimental group is less than that of control group.it can be oberserved that the d...
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