| IntroductionGene polymorphism is a common phenomenon. Many cytokine genepolymorphisms resul t in varial ions of cytokine production, which directly affect the processes of immune response. In this connection, it has being a hot-point of immunology in recent years about cytokine gene polymorphisms arid their impacts on organ transplantation. Some cytokines , such as TNF- , T(!F- % INF- and IL-10 , which, namely "key cytokines" , have major influence on immune processes, are most studied, and researchesrelating to IL-10 consist of important parts.Part one: The IL-10 Gene Polymorphisms of Renal Allograft Recipients andDonorsObjective: To define the distribution of IL-10 genotypes among the renal allograftrecipients and donors.Materials and methods:1. Method of experiment: During SEP. 2002 to MAR. 2003, the DNA samples of both 52 I'irst-time recipients of cadaveric renal allograft and their 26 donors were collected from peripheral blood cell/spleen lymph cell. All samples were genotyped for their IL-10 gene polymorphismsusing polymerase chain reaction with specific sequence primer.2. Method of statistics: The genotype frequency, haplotype and phenotype frequency of IL-10 in recipient group and donor group were calculated. According to their haplotype and genotype, the higher (H), intermediate (I) or lower (L) production type was determined. The haplotype, genotype and production frequency were compared between two groups. With the help of SPSS 11.0 and EXCEL2000 software, associations were assessed using 2*2 contingency table analysis and the chi-square test, or Fisher's exact tests where appropriate.Result:1. All DNAsamples from recipients and donors were extracted successfully and the final DNA contents of samples met with the requirement for kit testing. The IL-10 gone polymorphisms of two groups were all genotyped.2. There was no higher haplotype and phenotype in either test or control group. For each frequencies of three polymorphism sites, -1082, -819and - 592, there were no statistics significance between two groups. Most P-values were large than 0.5, except for the result of site - 1082. At this site, the frequency of G (Guanylicacid) in control group was large than test group and P-value was 0. 2. For this reason, it was suspected that difl'erence about IL-10 gene polymorphisms exists between renal allograft recipients and normal population. The difference would become statistically significant if the samples areenlarged.Part two: Impacts of Donor and Recipient Interleukin-10 Gene Polymorphisms on Acute Rejection of Renal Allograft: A One-year StudyObjective: To observe impacts of donor and recipient IL-10 gene polymorphisms on renal acute rejection in the first year after transplantation. Material and methods:1. The observation objects were the recipients whose IL-10 gene polymorphisms were defined in Part one. No immune inducing regime was applied pro-operation. A triad immunosuppressive protocol (CsA/FK506+MMF/AZA+Pred) was appointed to each recipient. The rejection episodes and relating clinic data were documented during the first year after transplantation.2. To analyze the relationship between IL-10 gene polymorphisms and renal acute rejection, the rejection ratio (including single and multiple rejections) of different IL-10 genotype and phenotype were compared, grouped by donor, recipient and some donor-recipient combinations. Two known effecting factors, HLA-DR miss-match and cold ischemia time, were further analyzed to exclude their influence on the result. With the help of EXCEL2000 and SPSS 11.0, statistics analysis were performed using student-t test and chi-square or Fisher' s exactprobability lest, where appropriate. A P-value less than 0.05 was considered significant, whilst a value of less than 0. 1 indicated a trend. Results:1. Forty-nine recipients finished the one-year follow-up at the end. Acute rejections occurred to eighteen persons, seven of them had multi-rejections. No organ lost. Since no IL-10 higher phenotype in dono... |
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