The Studying On The Antitumor Active Components Of Marine Streptomyces Albogriseolus A2002

In the present dissertation, 236 Actinomycete strains were isolated from 24 marine sedment samples collected from JIAODONG byland .They were all tested at the based of screening program for cell cycle inhibitors using tsFT210 cell line. After retested two times there were 9 strains with stable activity, the positive data arrived 3.9%.Among these 9 active strains 3 strains exihited Go/G1 phase inhibitory activity ,5 strains showed G2/M phase inhibitory activity and 1 strains showed cytotoxic activity. Strain A2002 with strong and stable G2/M phase inhibitory activity was selected as the target strain after a series of pre-experiments and the fermentation time was 7 days with the highest activity and largest mycelium amount. A2002 was identified as Streptomyces albogriseolus.Guided by cell cycle inhibitory activity in a mouse cdc2 mutant cell, tsFT210,the ethyl acetate extraction of mycelium was the active part. Successive purification of the active part by VLCX ODS, Sephadex LH-2CK HPLC ,led to the isolation of 5 compouds .By means of spectroscopic method according to their physicochemical properties,they were two kinds of compounds. One was tricyclacetalactonin and were named 5-hydroxyl-tricyclacetalactonin(l), tricyclacetalactonin (2) respectively,the other was cyclopeptides and named cyclo(-Pro-Ala)(3) - cyclo(-Ala-Val)(4) , cyclo(-Pro-Tyr)(5) ?We used the SRB on the tsFT210 cancer cell line to test the cell proliferation inhibitory activity of compound 1 and 2 .Also the bioactivities of compound 1 and 2 on ts FT210 cell line were evaluated with the aid of morphologic study and flow cytometry analysis. The experimented results exhibited that compound 1 and 2 had the cell cycle inhibitory activity , apoptosis inducing activity and cyctoxic activity.And their activites represented amount-effect relation.At low dose compound 1 and 2 were G2/M phase inhibition ,at middle dose apoptosis inducing and at hige dose cytotoxic.The bioactivity of compound 1 was stronger than compound 2.Compound 3,4,5 had no obvious antituomor activity against the tsFT210 cancer cell line, compoud 2 was identified as new compound.