| Objective: To research the angiogenesic effects and mechanism of G-CSF on experimental acute myocardial infarction rats .Methods: 50 rats were randomly divided into A,B,C and D groups. Group A (n=5): control ; Group B (n=5): G-CSF ; Group C(n=20): AMI ; Group D (n=20): G-CSF+AMI .Animals of group C and D were sacrificed in batches on 1, 5, 9 and 14 days postoperatively . Blood samples and myocardial specimeus were obtained for experimental study. G-CSF was given consecutively 5 days by hypodermic injection (10ug· kg-1·d-1). A freeze-thaw injury model was used to induce AMI . Vascular endothelial growth factor(VEGF) in bood was measured by ELISA. With the use of immuno-histochemical staining , VEGF and neovessel density in myocardial infarction area were measured .Results: Compared with control , blood concentration of VEGF in group B , C and D were significantly increased (all with P<0.05). At 1 , 5 , 9 and 14 days after operation ,the increase of blood VEGF concentration in group D were higher than that in group C. Also,the same appearance were observed on VEGF expression and angiogenesis in myocardial infarction area between group D and C.Conclusions: Hypodermic injection of G-CSF may cause an enhanced angiogenesis of acute myocardical infarction tissue in experimental rats . The angiogenesic effects of G-CSF are obtained partly by upregulation of local myocardial tiusse and blood VEGF expression .
|
PDF Full Text Request