Experimental Study On Treatment Of Endometriosis With Xiao Chai Hu Tang In Rats

Objective: To investigate the effects of Xiao Chai Hu Tang (XCHT) on endometriotic tissue (EOS) and explore the probable mechanisms by means of the Endometriosis (EMS) rat model.Methods: (1) The rat EMS model established by surgical operation is divided into groups to give different concentration XCHT and Danazol to cure 4 weeks. (2) By observing and measuring EOS' volume before and past treatment, to explore the inhibition of XCHT and Danazol. (3)height of endometrial epithelium(H) , volume of glandular nucleus(V) and volume integrated optical density(Viod) of glandular nucleus in EOS were investigated by the morphometry method.(4) Ultrastructural change in EM and EOS of each group were observed by electron microscopy. (5)Caspase-3 and Fas protein expression in EM and EOS of each group were observed and analyzed by immunohistochemistry method.Results: (1) The rat models of surgically induced endometriosis was established successfully.(2)Compared with control group , the EOS'volume of middle or high-concentration XCHT and Danazol group was obviously reduced(P<0.01).(3)the diminution of the H and V and Viod in the middle or high-concentration XCHT and Danazol groups were more significant than the control group(P<0.01).(4) the glandular cell in the endometrial implant after therapy with middle or high-concentration XCHT showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. (5)The protein expression of Caspase-3 and Fas in control group's EM were higher than it EOS; while XCHT group was on the other way round and the difference was not obvious in Danazol group.Conclusions: (1)The abnormal apoptosis level play a important role in Endometriosis (2)XCHT could inhibit the growth and proliferation of EOS in rat EMS model. (3) The probable mechanisms that XCHT inhibit EMS was that cell apoptosis and necrosis had induced by XCHT immuno-modulation.