| Object: To study the effect of preservation through hepatic artery and washing biliary tract on biliary tract cell of rats donor liver in cold preservation.Methods: With portal vein of donor livers perfused through by UW solution in every group, perfuse through hepatic artery and wash biliary tract by different methods, then observe the change of biliary tract cell on different time in the cold preservation.Resulds: There are no obvious changes under different light microscope and electron microscope after 4 hours in cold preservation. After 8 hours in cold preservation, with portal vein and hepatic artery of donor liver of rats perfused through by UW solution, two groups of biliary tract cell which perfused by UW solution and Ringer' s solution changed mostly less than other groups, and there is no obvious difference between them. After 12 hours in cold preservation, the change of the group whose portal vein and hepatic artery perfused through by UW solution and biliary tract washed by UW solution is least.Conclusions: perfusing hepatic artery and washing biliary tract benefit the preservation of biliary tract cell. In 8 hours if you can clear choline or lower the density of choline, you can get the same effect, butwith the cold preservation lasting, we can get better effect of preservation of biliary tract cell when washing biliary tract by UW solution. |
