| Background and objectives: Bronchial asthma (abbreviating asthma) is a chronic disease that seriously threaten human health over the world, it is a kind of chronic inflammation of airway, which is induced by a series of inflammation mediators, such as mast cells, eosinophils , T lymphocytes and so on. Recently, with the development of immunology and molecular biology, study of pathogenesis of asthma from molecule to.gene Some new inflammtion mediators become focus. Cytokine is a messenger of interaction of intercells, it is secreted by living immune cell and small molecule polypeptide of mediating and regulating immune reaction.Iterleukin-thirteen(IL-13) is a kind of cytokine, secreted by living CD"1" helping T cell(TH2), recently found involving in formation of chronic inflammation of airway in asthma. Squamous cell carcinoma Antigen(SccAg) is a new type of related tumor antegens, is cysteine and serine protease inhibitors, study indicate SccAg shows the highest expression all of genes induced by IL-13, therefore, it is supposed that SccAg may play some role in the pathogenesis of asthma. United measuring serum IL-13, SccAg concentration is useful for monitoring development and remission, diagnosis and theraphy, the study has not been reported until now in China.Bronchialitis is a kind of acute down respiratory infections, epidemicology indicate children who suffered Respiratory synthetic virus(RSV) bronchialitis during childhood, 50%~70% of them will occur recurrent wheeze, even asthma. Cough variant asthma(CVA) is a main kind of disease of chronic cough in children, onlysymptom is chronic cough out of classic wheeze of asthma, it is easy to disdiagnosis and omitting diagnosis, it threaten children health seriously. It is not clear of the relation among bronchialitis, CVA and asthma until now. So it is significant from pathogenesis to inquire the relation among them, the study has not been reported all over the world.In the experiment, we use enzyme-linked immunosorbent assay(ELISA) to measure serum IL-13, SccAg and immunoglobulin E(IgE) levels in 32 children with asthma, 25 children with CVA, 36 children with bronchialitis and 33 normal children, for knowing variances and significances of serum IL-13, SccAg in children with asthma and preliminary inquirement to relations among asthma, bronchialitis and CVA, to avoid bronchialitis becoming asthma, disdiagnosis and omitting diagnosis of CVA.Materials and Methods: Using ELISAto measure serum IL-13, SccAg and IgE levels of 26 children with asthma in acute phase, 32 children with asthma in remission phase, 25 children with CVA, 36 children with bronchialitis, 40 children with pneumonia and 33 normal children, age, sex and so on are not significant analyzed by statistics(P>0.05). Using SPSS 11.0 statistical software , mean and standand( x s) indicate results, every group parameters conform to normal distribution, sample averages of every group are analyzed after analysis of even variance, after that using t and F test, using linear regression, linear correlation analyse, P value less than 0.05 indicate statistical significance.Results: l.Serum IL-13(147.01 23.79)ng/l and SccAg(3.01 0.37)ng/ml concentration are significantly higher in acute phase of asthma than in period of remission(86.08 20.88)ng/l, (2.46 0.37)ng/ml (P<0.001), whereas period of remission and control group(77.27 18.16)ng/l, (2.29 0.34)ng/ml are not significantly different(P>0.05);.Serum IgE concentration in acute phase of asthma(659.53 70.51)KU/L are significantly higher than in period of remission(414.62 77.05)KU/L, furthermore, they are significantly higher than control group(151.67 70.17) KU/L (P<0.001), view in table 1.2. Serum IL-13(159.17 21.96)ng/l, SccAg(3.26 0.30)ng/ml andIgE(711.53 56.59)KU/L concentration in medium, serious asthma are significantly higher than light asthma(139.41 22.22)ng/l, (2.84 0.31)ng/ml, (627.03 58.61)KU/L (P<0.01),view in table 2.3. Serum IL-13(80.74 18.08)ng/l, SccAg(2.31 0.35... |
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