Association Between 469K/E Polymorphism Of The Intercellular Adhesion Molecule-1 Gene And Atherosclerotic Thrombotic Cerebral Infarction

Objective Cerebral stroke has already become the third most common cause of dead diseases, atherosclerotic thrombotic cerebral infarction(ATCI) is to above half of cerebral stroke.So prohibition from atherosclerosis remains a challenge. Recent studies have found that intercellular adhesion molecule-1(ICAM-1) plays a crucial role in lumphocyte migration and activation,and is considered important in the pathogenesis of atherosclerosis. K469E is a common polymorphism of the ICAM-lgene with potential functional significance.The aim of the present case-control study is to evaluate the association between this polymorphism and atherosclerotic thrombotic cerebral infarction.Methods we conducted a case-control study of 53 hospital cases with ATCI(male 36,female 17.mean age 63.58+7.71 years, age range 45-80 years) and randomly selected healthy control subjects without evident cerebral arterial disease(male 46,female 25.mean age 62.80+7.65, age range 45~80).Those who had skeptical informations of infection and embolism were excluded.The ICAM-1 K469E polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).TC,TG. and FBG levels were estimated by autoanalyzer.Results (1)In the ATCI group and control group the distribution of allele frequency was K: 0.37 and 0.24; E: 0.63 and 0.76, respectively.There was significant difference between them (x2=4.824, P=0.035) .In total specimen, allele frequencies of K and E were 0.29 and 0.71, respectively .The distribution of the ICAM-1 K469E polymorphism in above groups was EE genotype: 21% (11/53), 7%(5/71) ,respectively; EK genotype: 32% (17/53), 34% (24/71), respectively; KKgenotype: 47% (25/53), 59%(42/71) respectively. The EE genotype was significantly more common in patients than controls (P=0.031).The distribution of genotype frenquencies was in Hardy-Weinberg equilibrium.(2) Logistic regression analysis revealed that hypertension and diabetes mellitus were the independent risk factors of ATCI.Conclusions (1)The present study suggests that there is significant association beteewn the ICAM-1 K469E polymorphism and ATCI in Chinese Han ethnic group.(2) hypertension and diabetes mellitus are the independent risk factors of ATCI.