| Objective: To establish a model of marrow stromal cell cultured in vitro and to investigate more in details about its characteristics. Methods: Bone marrow was obtained by aspiration from healthy rabbits, mononuclear cells were then isolated by centrifugation in Ficoll density gradient. Cells were seeded by 1 × 106/ml and cultured in 1640 medium containing 15% fatal bovine serum, Cells were examined under inverted microscope everyday and attachment and confluence time as well as the proliferation were then obtained. Results: Primary cultured cells began its attachment about 24-36 hours after been seeded, and confluenced after about two weeks.Subcultured cells confluenced after a week. The logarithmic phase of growth of passage1 was 2-5 days, and the population doubling time obtained from the growth curve was 24 hours. About 98% subcultured cells adhered to the wall in 12 hours. Conclusion: Cultured cells by this means take on typical fibroblast-like configuration and can be used as seeded cell for bone tissue engineering. |
PDF Full Text Request