| By using gradual concentration chromatography to isolate and purify the roots of Rumex Japonieus Houtt ,we got three bioactive constitutes ,named S1,S2,S3。 The following internal Standard TLC,HPLC-MS and Ft-IR spectrumscopy data prove out that S1 is Chrysophanol ;S2 is the isomeric of Chrysophanol;S3 is emodin.Sequently, the antimicrobial pharmacology comparison show that S1,S2,S3 individually have significant microbial inhibit bioactivity in four different bacteria :Escherichia.Coli,Bacillus.Subtilis,Staph.Aureus,Candida.Aibicans . |
