| Purpose: The malignant hematologic disease is a kind of malignant tumour anddisease of the hemopoiesis system, the incidence of the disease takes the first place in teenagers' malignant tumour, and it threatens the human health seriously. It is characterized by malignant unusual hyperplasia and soading of cells in medulla ossium and other tissues, which can damage the normal hemopoiesis function and the mechanism of blood coagulation fibrinolysis and immunology. The immune function of the organism has close relations with emergence and development of the rumour. When host's immune function is low or restrained, the incidence of tumour increases, and in the growth of the tumour, the patient's immune function is suppressed further. The malignant blood disease patient's immnue function is closely related to prognosis., ACA is an autoantibody, which is united in two ester base with negative electrics at phosphatide molecule phosphoric acid. It is significant in monitoring the body's immune function. ACA's target antigen is the negative cardiolipin in the blood platelets and the membrane of endothelium. It influences various reaction systems depending on cardiiolipin in the course of blood coagulation, disruptiung the function of blood coagulation and fibrinolysis. In addition, ACA's combination with the phosphatide of the membrane in the blood platelet increases the phagocytosis and destruction of the blood platelets by monocytes, reducing the blood platelets. At the same time ACA impels the blood platelet to be activated, and thus the thrombus forms easily, reducing the blood platelets further and causing the clinical manifestation of bleeding in the majority of malignant blood diesease patients. In order manifestation of bleeding in the majority of malignant blood disease patients. In order to study ACA's relation with the machanism of blood coaugulation fibrinolysis and immunology malignant blood disease patients, we measure the incidence of ACA, coagulation and fibrinolysis indexes, such as plasma fibrinogen, Ddimer , serozyme fragment 1+2, plasminogen, etc, and immune indexes, such as serum IgG,IgM,C3,C4,circulating immune complex,etc.through statistic analysis we investigate their relations and thus assess the dangerous degree of hemorrhage and immune state inmalignant blood disease patients, which can reduce patients' mortality associated with chemotherapy and improve overall survival rate. So ACA has great clinic practical application value.Methods: 1 Research object, selected from the outpatients and inpatients withmalignant blood diseases of the affiliated hospitals of Heze medical college and Qingdao university medical college. 2 control, selected from 100 healthy students of Heze medical college. 3 The laboratory measurement. 3.1 The plasma preparing, collect the vein blood, anticoagulated with the sodium citrate of 0.13mol/L. after centrifugation at 2500*g fortwenty minutes, collect upper plasma and place it in -30癈 environment. Measure it in one month. 3.2 The scrum preparing, collect vein blood after cultivated in 37癈 environment,centrafugating at 2500*g for twenty minutes. Draw serum and put it in -30 癈environment for measuring. 3.3 ACA measurement, measure serum IgG,IgA,IgM Mode ACA separately by ELISA. Each sample is measured for 2times. The second time is underwent one month after the first. Positive results are judged by ACA combination index upper limit * standard product A value. The reagent is purchased in the sun biotech company of Fujian. 3.4 The measurement of blood coagulation and fibrinolysis indexes. The plasma fibrinogen (Fg), Ddimer(DD), serozymel+2(f1+2) and plasminogen are measured by Sysmex CAI500, Fg is measured by clauss, DD by immunonephelometry, f1+2 by ELISA. The reagent is purchased in Dade Behring Company. 3.5 Determine the immune index. Adopt the immunonephelometry to determine serum IgG, IgA, IgM, C3, C4. Immune complex is measured. 3.6 Statistic analysis. Three immunoglobulin (Ig) of ACA and blood coagulation, fibrinolysis and result of immune funct...
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