Studies On The Isolation, Purification, Identification And Function Of Antibacterial And Anticancer Active Components Of Allium Plant-Allium Chinense

Allium chinense is a kind of plants of Allium , which has important role in inflammation and health care in the traditional medicine. But it is lack of scientific proof, furthermore at present it is lack of analysis of quality and quantity to the effective medical components of Allium chinense. The isolation and purification of effective components of Chinese herb, for example Allium chinense, must accord with the standard of criterion of international markets as the development of science and technique and the improvement of people health level, we hope we can industrialize the product of Allium chinense, so we can increase the competition in the international medicine market.1. The isolation and identification of antibacterial and anticancer components of Allium chinenseThe effective components of Allium chinenses were extracted by Dichloromethane after they were crushed and enzymed. The sample was isolated by sephadex G-25 gel filtration column after concentration. Then bioactivity was assayed by Candida albicans. The chemical components were isolated and identified by GC-MS. GC determined the relative percentage content of the extract, nine kinds of components were separated, identified and accounted for about 82.12%, the major compound was propenyl methyl disulfide.2. The physical and chemical character of antibacterial and anticancer components of Allium chinenseAllium chinenses had antibacterial property to Shigella dysenteriae and Candida albicans. We utilized Uv/vis spectrophotometer to scan the sample after the effective componets were purified by sephadex G-25 gel filtration column, we found that the specific peak was near 237nm. So we could determine the quantity of the sample by absorbance A. Theeffective componets from elution solution of A Ilium chinenses had strong inhibition to HepG-2 cells and Hela cells, but the elution solution had better inhibition to HepG-2 cells than Hela cells. The anticancer effect of supernate was much lower than the elution solution. The temperature had influence on the effective components of elution solution, we found the maximal absorbance peak at 50℃, then the absorbance peak droped with the rise of temperature, the bioactivity disappeared at the same time. 3. Analyzing of biological function of antibacterial and anticancer components of Allium chinenseFCM analyzed the HepG-2 cells and Hela cells which were treated by the elution solution of Allium chinenses. The two kinds of cancer cells did not induce apoptosis after they were treated by elution solution, but the cells changed their cell cycle distribution. We did not find the DNA ladder in the electrophoresis of total DNA. Then we determined the influence on the enzyme ativity of papain, cellulase and proteinase K treated by elution solution of Allium chinenses. We found the enzyme activity of papain was improved by low concentration and inhibited by high concentration of elution solution, but the same concentration had no influence on the enzyme activity of cellulase and proteinase K. All the things proved that the elution solution of Allium chinenses maybe regulated enzymatic activity of SH containing enzymes by thiol-disulfide exchange reactions.