| ObjectiveMercury is a kind of toxic heavy metal that seriously contaminates the environment and mainly comes from the industrial producst. Kidney is the key organ in which inorganic mercury is transported, accumulated and expresses the toxici-ty. Mercury ion can combine tightly to the thiol of proteins in the kidney, which is due to accumulation of mercury in the kidney. 2,3-dimercaptopropane-l-sul-fonate (DMPS) is the chelator that can bind to the mercury. DMPS can fetch the mercury that combines to enzymes and restores the activity of enzymes. D-penicillamine ( DPA ) is the metabolic product of penicillin and belongs to the a-mino acid that contains the thiol. Both DMPS and DPA can combine to the mercury and form the indiscerptible and nontoxic chelating compound, which discharge out of body through the urine and feces. This study aims at firstly that to observe the effect of different dosage mercury doing harm to kidney in the Wistar rats and to determine the acute toxic dosage of mercury. Meanwhile we have an interruption with DMPS and DPA during the intoxicating experiment so that to discuss the preventive effect on DMPS and DPA mitigating the neprotoxicity and oxidative stress damage, which provide the academic base for precaution and healing when acute mercury poisoning occurs.Method48 Wistar rats obtained from the Experimental Animal Center of ChineseMedicine University, weighing 150 10 gram. The rats were housed at 22 2 Celsius degree with alternating light and dark. After an acclimation period of a week, rats were randomly assigned into 6 groups, which were respectively described as control group, 0.75, 1.5, 2.5mg/kg mercury group, DMPS prevention group and DPA prevention group. Every group contains 4 female and 4 male rats. During the experiment rats of control group were injected subcutaneously with 5 ml/kg body weight 0. 9% saline. And 0. 75, 1.5, 2.5 mg/kg mercuric chloride was respectively injected subcutaneously (sc) to rats of 0. 75 mg/kg, 1. 5 mg/kg and 2.5 mg/kg Hg group. Rats of DMPS and DPA prevention groups were in advance respectively given intraperitoneaUy ( ip) injection with 200mmol/kg body weight DPA and 200mmol/kg body weight DMPS, after 2 hours rats were injected subcutaneously in 2. 5mg/kg mercuric chloride. After intoxicated 12 hours, rats of all sorts were transfer to the metabolic cages to collect the urine. And 48 hours later, 5 ml of blood were collected by puncture into abdominal aorta under ether anesthesia. Both kidneys and livers were immediately removed. All samples were made to homogenate. During the experiment process all operations were run carefully under the iced condition.Then the livers and renal cortexes were hemogenated. Hg contents in the liver, renal cortex and urine samples were measured by cold atomic absorptive method. Urinary NAG activities were measured by the p-nitrohydroxybenzene colorimetry. Urinary ALP activities were measured by Jins method. Urinary protein contents were measured by Coomassie brilliant blue method. Urinary creati-nine levels were measured by picric acid colorimetry. BUN contents were measured by diacetyloxime method. GSH contents in the liver and renal cortex were measured by DTNB method. GSH-Px contents in the liver and renal cortex were measured by DTNB directing method. And MDA contents in the liver and renal cortex were measured by thiobarbituric acid colorimetry.ResultsAccompanied with increasing treated mercury dosage, the activities of urinary NAG and ALP, the contents of urinary protein and BUN in various treatedgroups go up. However all previous signs only in 2. 5mg/kg Hg group have a significant difference compared to the control. The activities of NAG and ALP and the contents of urinary protein and BUN, in the groups of all sorts treated with mercury and DMPS preventive group, decrease significantly. Urinary NAG activity and urinary protein content in the DPA preventive group go down significantly compared to the 2.5mg/kg group. However there are no variance on the ALP activity and BUN content in... |
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