The Role Of Interleukin-10 And Nuclear Factor-κB In Experimental Acute Necrotizing Pancreatitis

Objective: To investigate the role of interleukin-10(IL-10) and nuclear factor- K B(NF- K B) in L-arginine(L-Arg)-induced acute necrotizing pancreatitis (ANP). And to assess the early effects of IL-10 on pathophysiological changes in rats with ANP.Methods: Ninty-two SD rats were divided into three groups. ANP were induced in group A and group I by injecting intraperitoneally three times with 6% L-Arg (3# 1.0mg/g) in an interval of 1 hour. Group control received saline intraperitoneal injection only. Then group I was treated with 10,000 unites of intraperitioneal rhIL-10 at 2, 5 and 8 hours after the L-Arg induced. Rats were killed at 4th,12th,24th and 36th hours after the last time of L-Arg injection. The subunit p65 of NF- K B in pancreatic cell nuclear extractive and serum levels of amylase were assayed with Enzyme-linked immunoabsordent assay(ELISA). Pancreatic tissues were fixed in formalin. Histological characteristics were scored according to Rongione's standard.Results: The activity of NF- K B in group A was significantly higher than than in group control( P<0.05) .In comparison to group A, serum levels of amylase in group I were significantly decreased at 4th, 12th, 24th and 36th hours and histological scores in group I were significantly reduced at 12th, 24th and36th hours after rhIL-10 administration (all P<0.05). In group I, the activity of NF- K B was significantly suppressed compared to group A at 4th, 12 th, 24 th and 36 th hours (all P<0.01).Conclusions: The results suggest the activation of NF- K B play an important role in early phase of ANP. IL-10 can suppress the activation of NF-K B, decrease serum levels of amylase and reduce pancreatic tissue damage.