Effects Of Botulinum Toxin A On Rat Model Of Benign Prostate Hypertrophy

Abstract Objective: To investigate effects of botulinum toxin A on rat model of benign prostate hypertrophy.Methods: 64 male Wistar rats were divided into sham operation group(n=12) and model group(n=52). After castrated,52 rats of model group were injected subcutaneously testosterone propionate 4mg/kg.d to induce BPH;12 rats of sham operation group were injected subcutaneously the same dose of olive oil .After 1 week, the 4 model rats were randomly killed.The structure of prostates were observed by light microscopy.It proved that rat model was successful.The other 48 model rats were randomly divided into high dose group,medium dose group,low dose group,negative control group.The high, medium,low dose group received respectively 15u/0.1ml ,10u/0.1ml ,5u/0.1ml BTX-A injected into the prostateï¼›negative control group undergent 0.1ml NS injection into the prostate at the same position. After injections, the high,medium,low dose group,negative control group rats were injected subcutaneously testosterone propionate 4mg/kg.d for four weeks; sham operation group was injected subcutaneously the same dose of olive oil .The partly rats of the groups were killed randomly at 2,4 weeks. The prostates,bladders,hearts,kidneys,livers and hearts were removed.The structural changes of prostates,bladders,hearts,kidneys,livers and hearts were observed by light microscopy.The prostate weigh,prostate index(PI) were calculated.Morphometric changes of glandul areas,interstitial areas were semi-quantified by image analysis system.The expression of bax,fas,bcl-2 protein were analysed by means of immunohistochemical technique.All data were analysed by SPSS.Results:At two weeks,compared with negative control group, prostate weigh,PI,glandular areas,bcl-2 protein of high,medium,low dose group were significantly decreased(P<0.05),the expression of bax,fas protein significantly increased (P<0.05), interstitial areas relatively increased(P<0.05).At four weeks,compard with negative control group,prostate weighs,PI,glandular areas,bcl-2 protein of high,medium dose group were significantly decreased (P<0.05),the expression of bax,fas protein significantly increased(P<0.05), interstitial areas relatively increased(P<0.05),glandular areas of low dose group remarkablely differences(P<0.05).There were insignificantly differences between low group and negative control group in interstitial areas (P>0.05).There was insignificantly differences between each index of two weeks and four weeks in sham operation group.Conclusions: BTX-A can induce glandular atrophy of the rat model of BPH. Applications of BTX-A therapy could be candidated for the new treatment of BPH.