The Study Of Culture Human Skin Fibroblast On Allogeneic Acellular Dermal Matrix

Objective: To explore the methods of isolation , culture of human skin fibroblast in vitro, to observe the biological features in order to collect the normal table growth of fibroblast in vitro; and to explore the growth of human skin fibroblast on allogeneic acellular demal matrix.Methods: 1 ..To isolate and culture the human skin fibroblast. 2. To observe the biological features of fibroblast. 3 The features of cells seeded on the surface of acellular dermal matrix were observed by ways of lights and scanning electro microscopy. Results: 1. By the digests of DispaseD and Collagenase, we can isolate the pure fibroblast fast and efficiently, to confirm the DispaseD and Collagenase is better methods of culture human skin fibroblast. 2. The culture of human skin fibroblast in vitro has a stable biological features. 3. The fibroblast were seeded on the surface of acellular demal matrix proliferated. Conclusion: Normal human skin fibroblast that are isolated by usingDispase and Collagenase were able to proliferate and confluent, the proper seeding concentration of human skin fibroblast were 2X105 /75 cm 2flask. The fibroblast seeded on the surface of ADM can proliferate as a result the living dermal substitute was constructed.