Construction The Tissue Engineering Bladder By Autologous Muscle Derived Satellite Cells And Xenogeneic Bladder Acellular Matrix

Part I: Primary culture and purification of skeletal muscle derived satellite cells for tissue engineering applications[Objective] To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique for tissue engineering applications.[Methods] The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous Percoll density gradient centrifugation technique. The cells were observed under invert microscope and scanning electron microscope. The degree of purification was examined by celluar immunochemical stain. The growth curve was tested by MTT assay. Cell mitotic index were mensurated to realize the characteristic of cell growth.[Results] Over 90% satellite cells were harvested by incontinuous density Percoll gradient centrifugation technique, in contrast to which, only 30-40% cells were harvested by different adhesion time. Morphological observation accorded with satellite cells. The growth curve indicated that the cells have a good state of growth and better ability of proliferation.[ Conclusion ] The high purification satellite cells could be obtained by incontinuous density Percoll gradient centrifugation technique. It is a good method to culture seed cells for tissue engineering applications, especially for tissue engineering bladder. Through which high quality and enough quantity seeds could be obtainted only within a relatively brief time.Part II: Research the characteristic and preparation of porcine bladder acellular matrix graft for tissue engineering applications [Aabstract] Objective To investigate the preparation method of porcine bladder...