| Background: Chlamydia peumonia ( C.pn) is the important pathogen which in 1989 named one kind of respiratory tract infection , was the third kind of pathogenic chlamydia. C.pn , only a blood serum has the unique development cycle, the original body (EB) has infectiousness, mainly through the fat polysaccharide ( LPS) , the hot shock protein (hot shock protein, HSP), the main adventitial protein ( main out membrane protein, MOMP) and Sub-pathogenesis. The C.pn pathogenesis is widespread, may cause pneumonia, pharingolaryngitis, bronchitis, asthma and so on chronic respiratory tract infections, simultaneously also with coronary disease, atherosclerosis, myocardial infarction, endocarditis, close correlation and so on heart internal membrane inflammation. C.pn is very popular in each place of the world, thus received the people enormous attention. At present the C.pn laboratory inspection method has the etiology to inspect, the specificity nucleic acid examination, the specificity immune body determination and so on. But on clinical still not fast, convenient examination method, also not effective, feasible prevention measure. The microorganism parasites which as the special living cell in, the cell culture method of preserving separation to examine the C.pn long time, the operation is complex, is disadvantageous to the clinical fast diagnosis; But uses expands the spot increases DNAto gather the enzyme chain reaction technology ( PCR method), although has fast, simple, is desired specially, when carries on PCR examination should pay attention to the quality control, its accuracy easily specimen in impurity disturbs and experiments the personnel technical level influence, at present not suitably makes the conventional diagnosis. The nucleic acid hybrid mainly uses in PCR to expand increases the result to diagnose as well as the enhancement diagnosis sensitivity, but because tests the meter. The equipment request is higher , not yet directly uses in the clinical specimen examination. The specificity immune body determination, mainly is the immunity fluorescence mark technology is the present C.pn infection. The sensitive examination method, is recognized for is infects the serological diagnosis the golden standard method. In many countries and the medical unit, use the micro indirect immunity fluorescence (MIF) to examine the specificity immune body. The method has widely used in the epidemiology research and the clinical infection diagnosis. The MIF examination reagent uses the antigen is the C.pn membrane surface antigen. The literature material indicated, C.pn out membrane protein (OMP) has good immunity original, its immune body has the immunity protection. In addition, through genetic engineering fungus and so on backwoods coli obtains the reorganization protein, its immunity original good, also the entire production cost is lower, also may massively serve as the separation purification. Therefore, we test in the predecessor in the foundation, expands through PCR increases gains the C.pn membrane protein (OMP) the gene, has constructed C.pn OMP reorganization material particle pGEX-5X-3-OMP, and obtains the C.pn reorganization protein through the genetic engineering fungus, took the clinical examination reagent box the antigen, or uses by the new vaccine development and the development.Objective : Constructs Chlamydia pneumonia membrane surface protein (OMP) gene reorganization material particle pGEX-5X-3-OMP, and obtains C.pn through thegenetic engineering fungus the reorganization protein, took the clinical examination reagent box the antigen, or uses by the new vaccine development and the develoomentMaterials and Methods1. prepares the pGEX-5X-3 material particle: PGEX-5X-3 the material particle transformation backwoods coli DH5 alpha strain, amplified the positive clone, by alkali crack solution small amount preparation carrier.2. PCR amplification of C.pn OMP gene: Designs and synthesizes C.pn OMP gene sequevce to direct the thing, the directing beginnings and e...
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