Effect Of The Glutathione Redox State On Galn/LPS-induced Actue Liver Injury

Aim:To study the effect of the change of the glutathion redox state on Galn/LPS induced actue liver injury.Methods: In this experiments, 40 male mice were randomly divided into four groups. 1. liver injury group mice were treated with LPS plus D-galactosamine(Galn);2. DEM plus Galn/LPS groupe were treated with DEM before 1 hour of the Galn/LPS injection;3. DEM groupe 4. controlgroup .All groups were observed the level of ALT,TNFa,GSH,MDAand kupffer 'cells activity. And KCs isolated to assess function of KC'sphagocytose and ability of KCs' secretion after DEM injection.Results:. Afterl. Shours, Male mice were treated with LPS plus D-galactosamine(Galn) have not liver enzyme release changes,but has the high excent of kupffer cells activity and oxidative stress Western blot showLPS plus Gain can affect the IB degradation and nuclear factor-B activity. Further work show DEM can affect modulate nuclear factor-KB activity the IB degradation and nuclear factor-KB activity. We conclude that redox manipulation through thiol oxidation DEM can deplete intracellularGSH, may represent a novel approach to preventing LPS-induced liver injury by modulating kupffer'cells activity, NF-кB transduction pathway.We conclude that redox manipulation through thiol oxidation DEMmay represent a novel approach to preventing LPS-induced liver injury bymodulating kupffer'cells activity, NF-кB transduction pathway.